RRC ID 60351
Author Mekala LP, Mohammed M, Chinthalapati S, Chinthalapati VR.
Title Pyomelanin production: Insights into the incomplete aerobic l-phenylalanine catabolism of a photosynthetic bacterium, Rubrivivax benzoatilyticus JA2.
Journal Int J Biol Macromol
Abstract Rubrivivax benzoatilyticus JA2 is a metabolically versatile bacterium, thrives on a wide array of organic compounds under different growth modes. Though genomic insights revealed the aromatic compound catabolic potential of strain JA2 under anaerobic/aerobic conditions, the studies are largely restricted to anaerobic metabolism. The previous study on phenylalanine metabolism in strain JA2 indicated melanin-like pigment production under aerobic conditions; however, characterization of pigment and its biosynthetic pathway is not explored. The current study aims at the characterization of pigment and elucidation of its biosynthetic pathway. Strain JA2 utilized l-phenylalanine as source of nitrogen under anaerobic/aerobic conditions but not as a carbon source. Strain JA2 produced a brown-pigment under phenylalanine-amended aerobic conditions. Spectroscopic and physicochemical analysis identified the purified brown-pigment as a melanin. Further, the genomic insights revealed the presence of a complete set of genes related to pyomelanin synthesis. Identification of key metabolites l-tyrosine, 4-hydroxyphenylpyruvic acid and homogentisic acid and their respective enzyme activities further supports the pyomelanin synthesis. Moreover, the precursors feeding, pathway specific inhibitor studies confirmed the pyomelanin synthesis in strain JA2. Our study revealed an incomplete catabolism of phenylalanine; absence of ring cleavage gene, homogentisate dioxygenase leading to homogentisate accumulation thereby pyomelanin synthesis in strain JA2.
Volume 126
Pages 755-764
Published 2019-4-1
DOI 10.1016/j.ijbiomac.2018.12.142
PII S0141-8130(18)34945-6
PMID 30572055
MeSH Aerobiosis Betaproteobacteria / growth & development Betaproteobacteria / metabolism* Cyclohexanones / chemistry Cyclohexanones / metabolism Genome, Bacterial Melanins / biosynthesis* Melanins / chemistry Mesylates / chemistry Mesylates / metabolism Phenylalanine / metabolism* Photosynthesis* Phylogeny Tyrosine / metabolism X-Ray Diffraction
IF 4.784
Times Cited 5
General Microbes JCM13220