RRC ID 60353
Author Joulak I, Finore I, Nicolaus B, Leone L, Moriello AS, Attia H, Poli A, Azabou S.
Title Evaluation of the production of exopolysaccharides by newly isolated Halomonas strains from Tunisian hypersaline environments.
Journal Int J Biol Macromol
Abstract Ten Halomonas strains were screened from different Tunisian hypersaline environments for the production of exopolysaccharides (EPS), characterized and identified basing on 16S rRNA gene sequencing. EPS production was therefore studied using two different culture media M1 (complex medium) and M2 (semi-complex medium). Selected isolates produced different EPS amounts ranging from 86 to 170 mg L-1 and 26 to 105 mg L-1 when grown on M1 and M2, respectively. The use of M1 encouraged stronger bacterial growth associated with greater EPS production compared to M2. Nevertheless, the highest EPS yield (YEPS/X) was observed for strains grown on M2. When cultivated on M1, all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose, glucose and arabinose being the main monomers. However, the produced EPS on M2 were characterized by heterogeneous monosaccharide profiles among the different species, mostly consisting of glucomannan that could be a versatile material used for many further applications.
Volume 138
Pages 658-666
Published 2019-10-1
DOI 10.1016/j.ijbiomac.2019.07.128
PII S0141-8130(19)33673-6
PMID 31344416
MeSH Chemical Phenomena Culture Media Environment* Geography Halomonas / classification Halomonas / physiology* Monosaccharides Phylogeny Polysaccharides, Bacterial / biosynthesis* Saline Solution, Hypertonic* Tunisia
IF 4.784
Times Cited 3
Resource
General Microbes JCM32863 JCM32864 JCM32865 JCM32866 JCM32867 JCM32868 JCM32859 JCM32860 JCM32862 JCM32858