RRC ID 60538
著者 Kato S, Itoh T, Yuki M, Nagamori M, Ohnishi M, Uematsu K, Suzuki K, Takashina T, Ohkuma M.
タイトル Isolation and characterization of a thermophilic sulfur- and iron-reducing thaumarchaeote from a terrestrial acidic hot spring.
ジャーナル ISME J
Abstract A deep-branching clade of Thaumarchaeota, conventionally called Terrestrial hot spring creanarchaeotic group (THSCG), is a missing link between thaumarchaeotic ammonia oxidizers and the deeper-branching non-ammonia oxidizers, such as Crenarchaeota and Candidatus Korarchaeota. Here, we report isolation of the first cultivated representative from the THSCG, named as NAS-02. Physiological characterization demonstrated that the isolate was a thermoacidophilic, sulfur- and iron-reducing organoheterotroph, which was supported by gene contents encoded in its complete genome. There was no evidence for ammonia oxidation by the isolate. Members in THSCG are likely thermophiles, and may play roles in degrading cell debris as a scavenger and in biogeochemical cycling of sulfur and iron in the hot environments, as suggested by the physiological characteristics of the isolate and the geographical distribution of the 16S rRNA gene sequences of THSCG in terrestrial hot springs and marine hydrothermal fields. Phylogenetic analysis suggests that the THSCG lineage represented by NAS-02 has gained the ability of sulfur reduction via horizontal gene transfer. Based on the phylogeny and physiology, we propose the name Conexivisphaera calidus gen. nov., sp. nov. to accommodate the isolate.
巻・号 13(10)
ページ 2465-2474
公開日 2019-10-1
DOI 10.1038/s41396-019-0447-3
PII 10.1038/s41396-019-0447-3
PMID 31171857
PMC PMC6775994
MeSH Archaea / classification Archaea / genetics Archaea / isolation & purification Archaea / metabolism* DNA, Bacterial / genetics Hot Springs / chemistry Hot Springs / microbiology* Hot Temperature Iron / metabolism* Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sulfur / metabolism*
IF 9.493
引用数 1
リソース情報
一般微生物 JCM31663