| Abstract |
A polyphasic approach was used to identify the novel actinomycete, strain 10-20SHSuT, isolated from the rhizosphere of the mangrove associated plants Suaeda maritima collected from Phetchaburi Province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism belonged to the phylogenetic cluster of the genus Nonomuraea and was most closely related to Nonomuraea soli YIM 120770T (98.1% sequence similarity), Nonomuraea endophytica YIM 65601T (97.3%) and Nonomuraea candida HMC10T (97.3%). The strain formed an extensively branched substrate and aerial mycelia. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, with galactose, glucose, madurose, mannose and ribose as the whole-cell sugars. The polar lipids were diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol, phosphotidylmethylethanolamine, two unidentified sugar containing phosphoaminolipids and an unidentified phospholipid. MK-9(H4) was a major menaquinone of the organism. The predominant cellular fatty acids were iso-C16:0, C17:0 and 10-methyl-C17:0. The G + C content of the genomic DNA was 71.9 mol%. On the basis of phenotypic characteristics, DNA-DNA relatedness and phylogenetic distinctiveness, strain 10-20SHSuT represents a novel species of the genus Nonomuraea, for which the name Nonomuraea suaedae sp. nov. is proposed. The type strain is 10-20SHSuT (=TBRC 8487T =NBRC 113448T).
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