| RRC ID |
60540
|
| 著者 |
Takuma M, Kuroha M, Nagano Y, Kaweewan I, Hemmi H, Oyoshi T, Kodani S.
|
| タイトル |
Heterologous production of coryneazolicin in Escherichia coli.
|
| ジャーナル |
J Antibiot (Tokyo)
|
| Abstract |
Coryneazolicin is a plantazolicin family peptide, belonging to linear azole-containing peptides (LAPs). Although coryneazolicin was previously synthesized by in vitro experiments, its biological activity has not been evaluated. In this report, the heterologous production of coryneazolicin was accomplished to obtain enough coryneazolicin for biological activity tests. The structure of coryneazolicin was confirmed by ESI-MS and NMR analyses. The biological activity tests indicated that coryneazolicin possessed potent antibacterial activity and cytotoxicity. Although antibacterial activity of plantazolicin was previously reported, cytotoxicity was newly found in coryneazolicin among plantazolicin type peptides. In addition, we revealed that coryneazolicin induced apoptosis on HCT116 and HOS cancer cell lines.
|
| 巻・号 |
72(11)
|
| ページ |
800-806
|
| 公開日 |
2019-11-1
|
| DOI |
10.1038/s41429-019-0212-x
|
| PII |
10.1038/s41429-019-0212-x
|
| PMID |
31366953
|
| MeSH |
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology
Antineoplastic Agents / chemistry
Antineoplastic Agents / metabolism
Antineoplastic Agents / pharmacology
Bacteria / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Escherichia coli / metabolism*
Humans
Peptides / metabolism*
Protein Conformation
|
| IF |
2.446
|
| 引用数 |
0
|
| リソース情報 |
| 一般微生物 |
JCM10395 |
