論文 - 詳細
| RRC ID | 60546 |
|---|---|
| 著者 | Goto S, Hokamura A, Shiratsuchi H, Taguchi S, Matsumoto K, Abe H, Tanaka K, Matsusaki H. |
| タイトル | Biosynthesis of novel lactate-based polymers containing medium-chain-length 3-hydroxyalkanoates by recombinant Escherichia coli strains from glucose. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Novel lactate (LA)-based polymers containing medium-chain-length 3-hydroxyalkanoates (MCL-3HA) were produced in fadR-deficient Escherichia coli strains from glucose as the sole carbon source. The genes encoding LA and 3-hydroxybutyrate (3HB) monomers supplying enzymes [propionyl-CoA transferase (PCT), d-lactate dehydrogenase (D-LDH), β-ketothiolase (PhaA), and NADPH-dependent acetoacetyl-CoA reductase (PhaB)], MCL-3HA monomers supplying enzymes [(R)-3-hydroxyacyl-ACP thioesterase (PhaG) and (R)-3-hydroxyacyl (3HA)-CoA ligase] via fatty acid biosynthesis pathway, and modified polyhydroxyalkanoate (PHA) synthase [PhaC1(STQK)] of Pseudomonas sp. 61-3 were introduced into E. coli LS5218. This resulted in the synthesis of a novel LA-based copolymer, P(LA-co-3HB-co-3HA). 1H-nuclear magnetic resonance (NMR) analysis revealed the composition of P(LA-co-3HB-co-3HA) to be 19.7 mol% LA (C3), 74.9 mol% 3HB (C4), and 5.4 mol% MCL-3HA units of C8 and C10. Furthermore, the recombinant E. coli CAG18497 strain carrying these genes, excluding the phaAB genes, accumulated P(92.0% LA-co-3HA) with a novel monomer composition containing C3, C8, C10, and C12. 13C-NMR analysis showed the existence of LA-3HA sequence in the polymer. The solvent cast film of P(92.0% LA-co-3HA) exhibited transparency similar to poly(lactic acid). |
| 巻・号 | 128(2) |
| ページ | 191-197 |
| 公開日 | 2019-8-1 |
| DOI | 10.1016/j.jbiosc.2019.01.009 |
| PII | S1389-1723(18)31170-8 |
| PMID | 30799088 |
| MeSH | Acyltransferases / genetics Acyltransferases / metabolism Alcohol Oxidoreductases / genetics Alcohol Oxidoreductases / metabolism DNA, Recombinant / genetics* Escherichia coli / genetics* Escherichia coli / metabolism* Glucose / metabolism* Lactic Acid / chemistry* Polymers / chemistry* Polymers / metabolism* Pseudomonas / genetics |
| IF | 2.032 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM10015 |