RRC ID 60570
Author Liu YH, Yamazaki W, Huang YT, Liao CH, Sheng WH, Hsueh PR.
Title Clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species with an emphasis on the subspecies of C. fetus.
Journal J Microbiol Immunol Infect
Abstract OBJECTIVES:This study was intended to investigate the clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species.
METHODS:From April 1998 to May 2014, 56 adults with bacteremia caused by Campylobacter species were evaluated. These Campylobacter species isolates were confirmed to the species level using 16S rRNA gene sequencing (all isolates) and multiplex PCR analysis (for C. fetus only). The performance of identification for Campylobacter species by the Bruker Biotyper MALDI-TOF MS was evaluated. The genetic relatedness of C. fetus isolates was analyzed by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE).
RESULTS:The leading underlying medical conditions of these patients were malignancy (46.4%), hypertension (35.7%), and liver cirrhosis (23.2%). The overall 30-day mortality rate was 5.4%. Using 16S rRNA sequencing analysis, 26 isolates of C. coli, 11 of C. jejuni, and 19 of C. fetus, including 15 C. fetus subsp. fetus and five C. fetus subsp. venerealis, were identified. Among the five C. fetus subsp. venerealis isolates recognized by 16S rRNA gene sequencing, only two isolates were C. fetus subsp. venerealis by multiplex PCR method. The Bruker Biotyper MALDI-TOF MS failed to correctly identify C. fetus subsp. venerealis isolates. MLST analysis of C. fetus isolates revealed three STs: ST20 (n = 12), ST11 (n = 5), and ST57 (n = 2), which were compatible with three major PFGE clusters.
CONCLUSION:Database expansion of MALDI-TOF MS for the correct identification of C. fetus to subspecies levels is needed. A novel clone of ST57-PFGE Cluster C of C. fetus subsp. venerealis was noted.
Volume 52(1)
Pages 122-131
Published 2019-2
DOI 10.1016/j.jmii.2017.07.009
PII S1684-1182(17)30149-4
PMID 28801089
MeSH Adolescent Adult Aged Aged, 80 and over Bacteremia / microbiology* Bacteremia / mortality Bacterial Typing Techniques Campylobacter / classification* Campylobacter / genetics Campylobacter Infections / microbiology* Campylobacter Infections / mortality Campylobacter fetus / classification Campylobacter fetus / genetics Child DNA, Bacterial / genetics Electrophoresis, Gel, Pulsed-Field Female Humans Male Middle Aged RNA, Ribosomal, 16S / genetics Risk Factors Sequence Analysis, DNA Young Adult
Times Cited 6
Resource
General Microbes JCM2528