Reference - Detail
| RRC ID | 60573 |
|---|---|
| Author | Sato S, Fukuoka T, Saika A, Koshiyama T, Morita T. |
| Title | A New Screening Approach for Glycolipid-type Biosurfactant Producers Using MALDI-TOF/MS. |
| Journal | J Oleo Sci |
| Abstract |
We applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) to screen for glycolipid-type biosurfactant (BS) producers. A crude extract of Pseudozyma antarctica, a well-known mannosylerythritol lipid (MEL) producer, was initially subjected to MALDI-TOF/MS. The spectrum of the extract showed the accumulation of diacylated MELs in culture. We then screened 80 environmental samples for BS-producing yeasts, and extracts from broth cultures of the selected five strains were examined using MALDI-TOF/MS. The results showed that all five strains produced MELs, whereas four strains also produced cellobiose lipids (CLs). By D1/D2 region sequence analysis, the MEL-producing strain was assigned to P. antarctica while the four MEL- and CL-producing strains were assigned to P. hubeiensis. These results demonstrate that MALDI-TOF/MS is a rapid and reliable tool to detect BS molecules in crude extracts of broth cultures to screen for glycolipid-type BS producers. |
| Volume | 68(12) |
| Pages | 1287-1294 |
| Published | 2019-1-1 |
| DOI | 10.5650/jos.ess19177 |
| PMID | 31787677 |
| MeSH | Glycolipids / analysis* Phylogeny Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods Surface-Active Agents / analysis* Ustilaginales / chemistry Ustilaginales / classification Ustilaginales / genetics |
| IF | 1.304 |
| Times Cited | 0 |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM10317 |