論文 - 詳細
| RRC ID | 60585 |
|---|---|
| 著者 | Mora-Lugo R, Stegmüller J, Mack M. |
| タイトル | Metabolic engineering of roseoflavin-overproducing microorganisms. |
| ジャーナル | Microb Cell Fact |
| Abstract |
BACKGROUND:Roseoflavin, a promising broad-spectrum antibiotic, is naturally produced by the bacteria Streptomyces davaonensis and Streptomyces cinnabarinus. The key enzymes responsible for roseoflavin biosynthesis and the corresponding genes were recently identified. In this study we aimed to enhance roseoflavin production in S. davaonensis and to synthesize roseoflavin in the heterologous hosts Bacillus subtilis and Corynebacterium glutamicum by (over)expression of the roseoflavin biosynthesis genes. RESULTS:While expression of the roseoflavin biosynthesis genes from S. davaonensis was not observed in recombinant strains of B. subtilis, overexpression was successful in C. glutamicum and S. davaonensis. Under the culture conditions tested, a maximum of 1.6 ± 0.2 µM (ca. 0.7 mg/l) and 34.9 ± 5.2 µM (ca. 14 mg/l) roseoflavin was produced with recombinant strains of C. glutamicum and S. davaonensis, respectively. In S. davaonensis the roseoflavin yield was increased by 78%. CONCLUSIONS:The results of this study provide a sound basis for the development of an economical roseoflavin production process. |
| 巻・号 | 18(1) |
| ページ | 146 |
| 公開日 | 2019-8-26 |
| DOI | 10.1186/s12934-019-1181-2 |
| PII | 10.1186/s12934-019-1181-2 |
| PMID | 31451111 |
| PMC | PMC6709556 |
| MeSH | Anti-Bacterial Agents / biosynthesis* Bacillus subtilis / genetics Bacillus subtilis / metabolism* Corynebacterium glutamicum / genetics Corynebacterium glutamicum / metabolism* Metabolic Engineering / methods* Riboflavin / analogs & derivatives* Riboflavin / biosynthesis Riboflavin / genetics Streptomyces / genetics Streptomyces / metabolism* |
| IF | 4.402 |
| 引用数 | 1 |
| リソース情報 | |
| 一般微生物 | JCM4913 |