論文 - 詳細
| RRC ID | 606 |
|---|---|
| 著者 | Sakakibara H, Kasahara H, Ueda N, Kojima M, Takei K, Hishiyama S, Asami T, Okada K, Kamiya Y, Yamaya T, Yamaguchi S. |
| タイトル | Agrobacterium tumefaciens increases cytokinin production in plastids by modifying the biosynthetic pathway in the host plant. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Agrobacterium tumefaciens infects plants and induces the formation of tumors called "crown galls" by integrating the transferred-DNA (T-DNA) region of the Ti-plasmid into the plant nuclear genome. Tumors are formed because the T-DNA encodes enzymes that modify the synthesis of two plant growth hormones, auxin and cytokinin (CK). Here, we show that a CK biosynthesis enzyme, Tmr, which is encoded by the Agrobacterium T-DNA region, is targeted to and functions in plastids of infected plant cells, despite having no typical plastid-targeting sequence. Evidence is provided that Tmr is an adenosine phosphate-isopentenyltransferase (IPT) that creates a new CK biosynthesis bypass by using 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBDP) as a substrate. Unlike in the conventional CK biosynthesis pathway in plants, trans-zeatin-type CKs are produced directly without the requirement for P450 monooxygenase-mediated hydroxylation. Consistent with the plastid localization of Tmr, HMBDP is an intermediate in the methylerythritol phosphate pathway, a plastid-localized biosynthesis route for universal isoprenoid precursors. These results demonstrate that A. tumefaciens modifies CK biosynthesis by sending a key enzyme into plastids of the host plant to promote tumorigenesis. |
| 巻・号 | 102(28) |
| ページ | 9972-7 |
| 公開日 | 2005-7-12 |
| DOI | 10.1073/pnas.0500793102 |
| PII | 0500793102 |
| PMID | 15998742 |
| PMC | PMC1174980 |
| MeSH | Adenine Nucleotides / metabolism Agrobacterium tumefaciens / enzymology* Alkyl and Aryl Transferases / metabolism* Arabidopsis / microbiology* Bacterial Proteins / metabolism* Blotting, Western Cytokinins / biosynthesis* Genetic Vectors Green Fluorescent Proteins Oncogene Proteins / metabolism* Organophosphates / metabolism Plant Tumor-Inducing Plasmids / genetics* Plant Tumors / genetics* Plant Tumors / microbiology |
| IF | 9.412 |
| 引用数 | 78 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00014 rpc00015 |