Reference - Detail
| RRC ID | 60616 |
|---|---|
| Author | Forster SC, Kumar N, Anonye BO, Almeida A, Viciani E, Stares MD, Dunn M, Mkandawire TT, Zhu A, Shao Y, Pike LJ, Louie T, Browne HP, Mitchell AL, Neville BA, Finn RD, Lawley TD. |
| Title | A human gut bacterial genome and culture collection for improved metagenomic analyses. |
| Journal | Nat Biotechnol |
| Abstract |
Understanding gut microbiome functions requires cultivated bacteria for experimental validation and reference bacterial genome sequences to interpret metagenome datasets and guide functional analyses. We present the Human Gastrointestinal Bacteria Culture Collection (HBC), a comprehensive set of 737 whole-genome-sequenced bacterial isolates, representing 273 species (105 novel species) from 31 families found in the human gastrointestinal microbiota. The HBC increases the number of bacterial genomes derived from human gastrointestinal microbiota by 37%. The resulting global Human Gastrointestinal Bacteria Genome Collection (HGG) classifies 83% of genera by abundance across 13,490 shotgun-sequenced metagenomic samples, improves taxonomic classification by 61% compared to the Human Microbiome Project (HMP) genome collection and achieves subspecies-level classification for almost 50% of sequences. The improved resource of gastrointestinal bacterial reference sequences circumvents dependence on de novo assembly of metagenomes and enables accurate and cost-effective shotgun metagenomic analyses of human gastrointestinal microbiota. |
| Volume | 37(2) |
| Pages | 186-192 |
| Published | 2019-2-1 |
| DOI | 10.1038/s41587-018-0009-7 |
| PII | 10.1038/s41587-018-0009-7 |
| PMID | 30718869 |
| PMC | PMC6785715 |
| MeSH | Bacteria / classification Computational Biology / methods Contig Mapping Gastrointestinal Microbiome Genome, Bacterial* Genome, Human Humans Metagenome* Metagenomics* Phylogeny RNA, Ribosomal, 16S / metabolism Sequence Analysis, DNA Species Specificity |
| IF | 31.864 |
| Times Cited | 51 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 671 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| General Microbes | JCM31252 JCM31253 JCM31267 JCM31265 JCM31268 JCM31257 JCM31261 JCM31262 JCM31260 JCM31255 |