RRC ID 60625
Author Koyanagi Y, Suzuki R, Ihara K, Miyagi H, Isogai H, Yoneyama H, Isogai E.
Title Intestinal Clostridium species lower host susceptibility to enterohemorrhagic Escherichia coli O157:H7 infection.
Journal Pathog Dis
Abstract Susceptibility to enterohemorrhagic Escherichia coli (EHEC) infection varies among humans. The intestinal microbiota seems to play an essential role in host defense against EHEC; thus, we hypothesized that indigenous bacteria, such as Clostridium ramosum and Clostridium perfringens, could influence the susceptibility to EHEC infection. To evaluate the effect of indigenous bacteria on EHEC infection, germ-free mice were precolonized with each indigenous bacterium, and then infected with EHEC O157:H7. Precolonization with C. ramosum or C. perfringens completely prevented death from EHEC infection througout a test period. Precolonization with C. ramosum also reduced the level of secreted Shiga toxin (Stx) 2 and prevented histopathological changes in the kidneys in a similar way to precolonization with Bifidobacterium longum, which is used as a model for preventing EHEC infection. In contrast, the mice precolonized with C. perfringens showed mild renal injuries. When evaluated using an in vitro co-culturing system, again C. ramosum inhibited the growth and Stx production of EHEC more potently than C. perfringens. These results indicate that C. ramosum and C. perfringens suppressed EHEC infection; however, the extent of their preventive effects differed. Therefore, the susceptibility to EHEC infection and its severity can depend on the functional bacteria present in the intestinal microbiota of individuals.
Volume 77(4)
Published 2019-6-1
DOI 10.1093/femspd/ftz036
PII 5538763
PMID 31344225
MeSH Animals Antibiosis* Clostridium perfringens / growth & development* Disease Models, Animal Disease Susceptibility* Escherichia coli Infections / microbiology Escherichia coli Infections / pathology Escherichia coli Infections / prevention & control* Escherichia coli O157 / growth & development* Firmicutes / growth & development* Gastrointestinal Microbiome Mice
Times Cited 0
General Microbes JCM1217 JCM1290 JCM1298