RRC ID 6063
Author Hayashida K, Kohno S.
Title Hybrid male sterility is caused by mitochondrial DNA deletion.
Journal Mol Biol Rep
Abstract Although it is known that the hybrid male mouse is sterile just like any other animal's heterogametic sex, the reason why only the male germ cells are impaired has yet to be discovered. TdT-mediated dUTP nick end labeling assay using a confocal fluorescence microscope and DNA fragmentation assay of hybrid testis indicated destruction of the mitochondrial DNA (mtDNA) rather than the nuclear DNA. Previously we reported that maternal mtDNA inheritance is through selective sperm mtDNA elimination based on the sperm factor and two egg factors, and expression of these three factors was recognized in the hybrid testis. It was thereby assumed that mtDNA destruction caused by the expression of maternal mtDNA inheritance system in male germ cells is implicated in the hybrid male sterility of mice.
Volume 36(6)
Pages 1365-9
Published 2009-7-1
DOI 10.1007/s11033-008-9321-5
PMID 18649010
MeSH Animals Chimera DNA Fragmentation DNA, Mitochondrial / genetics* In Situ Nick-End Labeling Infertility, Male / etiology Infertility, Male / genetics* Male Mice Microscopy, Fluorescence Sequence Deletion* Spermatozoa / ultrastructure Testis
IF 1.402
Times Cited 0
Mice RBRC00213