論文 - 詳細
| RRC ID | 60638 |
|---|---|
| 著者 | Sakanaka M, Hansen ME, Gotoh A, Katoh T, Yoshida K, Odamaki T, Yachi H, Sugiyama Y, Kurihara S, Hirose J, Urashima T, Xiao JZ, Kitaoka M, Fukiya S, Yokota A, Lo Leggio L, Abou Hachem M, Katayama T. |
| タイトル | Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis. |
| ジャーナル | Sci Adv |
| Abstract |
The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is primarily associated with the bifidobacteria-rich microbiota formation in breast-fed infant guts. Structural analyses of the solute-binding protein (SBP) of FL transporter-2 complexed with 2'-fucosyllactose and 3-fucosyllactose, together with phylogenetic analysis of SBP homologs of both FL transporters, highlight a unique adaptation strategy of Bifidobacterium to HMOs, in which the gain-of-function mutations enable FL transporter-2 to efficiently capture major fucosylated HMOs. Our results provide a molecular insight into HMO-mediated symbiosis and coevolution between bifidobacteria and humans. |
| 巻・号 | 5(8) |
| ページ | eaaw7696 |
| 公開日 | 2019-8-1 |
| DOI | 10.1126/sciadv.aaw7696 |
| PII | aaw7696 |
| PMID | 31489370 |
| PMC | PMC6713505 |
| MeSH | Adult Aged Bifidobacterium / metabolism Bifidobacterium / physiology* Biological Evolution Feces / microbiology Female Gastrointestinal Microbiome / physiology* Humans Infant Infant, Newborn Male Metagenome / physiology Middle Aged Milk, Human / metabolism Oligosaccharides / metabolism* Symbiosis / physiology* Trisaccharides / metabolism* Young Adult |
| IF | 12.804 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 22 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM1222 JCM15439 JCM31944 |