RRC ID 6063
著者 Hayashida K, Kohno S.
タイトル Hybrid male sterility is caused by mitochondrial DNA deletion.
ジャーナル Mol Biol Rep
Abstract Although it is known that the hybrid male mouse is sterile just like any other animal's heterogametic sex, the reason why only the male germ cells are impaired has yet to be discovered. TdT-mediated dUTP nick end labeling assay using a confocal fluorescence microscope and DNA fragmentation assay of hybrid testis indicated destruction of the mitochondrial DNA (mtDNA) rather than the nuclear DNA. Previously we reported that maternal mtDNA inheritance is through selective sperm mtDNA elimination based on the sperm factor and two egg factors, and expression of these three factors was recognized in the hybrid testis. It was thereby assumed that mtDNA destruction caused by the expression of maternal mtDNA inheritance system in male germ cells is implicated in the hybrid male sterility of mice.
巻・号 36(6)
ページ 1365-9
公開日 2009-7-1
DOI 10.1007/s11033-008-9321-5
PMID 18649010
MeSH Animals Chimera DNA Fragmentation DNA, Mitochondrial / genetics* In Situ Nick-End Labeling Infertility, Male / etiology Infertility, Male / genetics* Male Mice Microscopy, Fluorescence Sequence Deletion* Spermatozoa / ultrastructure Testis
IF 1.402
引用数 0
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
実験動物マウス RBRC00213