RRC ID 60642
著者 Carro L, Castro JF, Razmilic V, Nouioui I, Pan C, Igual JM, Jaspars M, Goodfellow M, Bull AT, Asenjo JA, Klenk HP.
タイトル Uncovering the potential of novel micromonosporae isolated from an extreme hyper-arid Atacama Desert soil.
ジャーナル Sci Rep
Abstract The taxonomic status, biotechnological and ecological potential of several Micromonospora strains isolated from an extreme hyper arid Atacama Desert soil were determined. Initially, a polyphasic study was undertaken to clarify the taxonomic status of five micromonosporae, strains LB4, LB19, LB32T, LB39T and LB41, isolated from an extreme hyper-arid soil collected from one of the driest regions of the Atacama Desert. All of the isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Micromonospora. Isolates LB32T and LB39T were distinguished from their nearest phylogenetic neighbours and proposed as new species, namely as Micromonospora arida sp. nov. and Micromonospora inaquosa sp. nov., respectively. Eluted methanol extracts of all of the isolates showed activity against a panel of bacterial and fungal indicator strains, notably against multi-drug resistant Klebsiella pneumoniae ATCC 700603 while isolates LB4 and LB41 showed pronounced anti-tumour activity against HepG2 cells. Draft genomes generated for the isolates revealed a rich source of novel biosynthetic gene clusters, some of which were unique to individual strains thereby opening up the prospect of selecting especially gifted micromonosporae for natural product discovery. Key stress-related genes detected in the genomes of all of the isolates provided an insight into how micromonosporae adapt to the harsh environmental conditions that prevail in extreme hyper-arid Atacama Desert soils.
巻・号 9(1)
ページ 4678
公開日 2019-3-18
DOI 10.1038/s41598-019-38789-z
PII 10.1038/s41598-019-38789-z
PMID 30886188
PMC PMC6423291
MeSH Anti-Infective Agents / isolation & purification* Anti-Infective Agents / therapeutic use Antineoplastic Agents / isolation & purification* Antineoplastic Agents / therapeutic use Cell Extracts Chile Desert Climate Drug Discovery Hep G2 Cells Humans Klebsiella Infections / therapy* Klebsiella pneumoniae / physiology* Liver Neoplasms / therapy* Methanol / isolation & purification* Micromonospora / physiology* Phylogeny Soil Microbiology Streptomyces / physiology Stress, Physiological / genetics
IF 4.011
引用数 5
リソース情報
一般微生物 JCM13248