RRC ID |
60648
|
Author |
Sumi S, Suzuki Y, Matsuki T, Yamamoto T, Tsuruta Y, Mise K, Kawamura T, Ito Y, Shimada Y, Watanabe E, Watanabe S, Toriyabe M, Takano Shiratori H, Ueda K, Takano H.
|
Title |
Light-inducible carotenoid production controlled by a MarR-type regulator in Corynebacterium glutamicum.
|
Journal |
Sci Rep
|
Abstract |
Carotenoid production in some non-phototropic bacteria occurs in a light-dependent manner to protect cells from photo-oxidants. Knowledge regarding the transcriptional regulator involved in the light-dependent production of carotenoids of non-phototrophic bacteria has been mainly confined to coenzyme B12-based photo-sensitive regulator CarH/LitR family proteins belonging to a MerR family transcriptional regulator. In this study, we found that bacteria belonging to Micrococcales and Corynebacteriales exhibit light-dependent carotenoid-like pigment production including an amino acid-producer Corynebacterium glutamicum AJ1511. CrtR is a putative MarR family transcriptional regulator located in the divergent region of a carotenoid biosynthesis gene cluster in the genome of those bacteria. A null mutant for crtR of C. glutamicum AJ1511 exhibited constitutive production of carotenoids independent of light. A complemented strain of the crtR mutant produced carotenoids in a light-dependent manner. Transcriptional analysis revealed that the expression of carotenoid biosynthesis genes is regulated in a light-dependent manner in the wild type, while the transcription was upregulated in the crtR mutant irrespective of light. In vitro experiments demonstrated that a recombinant CrtR protein binds to the specific sequences within the intergenic region of crtR and crtE, which corresponds to -58 to -7 for crtE, and +26 to -28 for crtR with respect to the transcriptional start site, and serves as a repressor for crtE transcription directed by RNA polymerase containing SigA. Taken together, the results indicate that CrtR light-dependently controls the expression of the carotenoid gene cluster in C. glutamicum and probably closely related Actinobacteria.
|
Volume |
9(1)
|
Pages |
13136
|
Published |
2019-9-11
|
DOI |
10.1038/s41598-019-49384-7
|
PII |
10.1038/s41598-019-49384-7
|
PMID |
31511549
|
PMC |
PMC6739363
|
MeSH |
Amino Acid Sequence
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Base Sequence
Carotenoids / metabolism*
Corynebacterium glutamicum / genetics*
Corynebacterium glutamicum / metabolism
Gene Expression Regulation, Bacterial / radiation effects*
Light*
Multigene Family / genetics
Promoter Regions, Genetic / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transcription Initiation Site
Transcription, Genetic / radiation effects*
|
IF |
4.011
|
Times Cited |
1
|
Resource |
General Microbes |
JCM1333
JCM1970
JCM1973
JCM2522
JCM1338
JCM1335
JCM1336
JCM15137
JCM17812
JCM15589
JCM19549
JCM18063
JCM1318
JCM9489
JCM1305
JCM11950 |