RRC ID 60648
著者 Sumi S, Suzuki Y, Matsuki T, Yamamoto T, Tsuruta Y, Mise K, Kawamura T, Ito Y, Shimada Y, Watanabe E, Watanabe S, Toriyabe M, Takano Shiratori H, Ueda K, Takano H.
タイトル Light-inducible carotenoid production controlled by a MarR-type regulator in Corynebacterium glutamicum.
ジャーナル Sci Rep
Abstract Carotenoid production in some non-phototropic bacteria occurs in a light-dependent manner to protect cells from photo-oxidants. Knowledge regarding the transcriptional regulator involved in the light-dependent production of carotenoids of non-phototrophic bacteria has been mainly confined to coenzyme B12-based photo-sensitive regulator CarH/LitR family proteins belonging to a MerR family transcriptional regulator. In this study, we found that bacteria belonging to Micrococcales and Corynebacteriales exhibit light-dependent carotenoid-like pigment production including an amino acid-producer Corynebacterium glutamicum AJ1511. CrtR is a putative MarR family transcriptional regulator located in the divergent region of a carotenoid biosynthesis gene cluster in the genome of those bacteria. A null mutant for crtR of C. glutamicum AJ1511 exhibited constitutive production of carotenoids independent of light. A complemented strain of the crtR mutant produced carotenoids in a light-dependent manner. Transcriptional analysis revealed that the expression of carotenoid biosynthesis genes is regulated in a light-dependent manner in the wild type, while the transcription was upregulated in the crtR mutant irrespective of light. In vitro experiments demonstrated that a recombinant CrtR protein binds to the specific sequences within the intergenic region of crtR and crtE, which corresponds to -58 to -7 for crtE, and +26 to -28 for crtR with respect to the transcriptional start site, and serves as a repressor for crtE transcription directed by RNA polymerase containing SigA. Taken together, the results indicate that CrtR light-dependently controls the expression of the carotenoid gene cluster in C. glutamicum and probably closely related Actinobacteria.
巻・号 9(1)
ページ 13136
公開日 2019-9-11
DOI 10.1038/s41598-019-49384-7
PII 10.1038/s41598-019-49384-7
PMID 31511549
PMC PMC6739363
MeSH Amino Acid Sequence Bacterial Proteins / genetics* Bacterial Proteins / metabolism Base Sequence Carotenoids / metabolism* Corynebacterium glutamicum / genetics* Corynebacterium glutamicum / metabolism Gene Expression Regulation, Bacterial / radiation effects* Light* Multigene Family / genetics Promoter Regions, Genetic / genetics Reverse Transcriptase Polymerase Chain Reaction Transcription Initiation Site Transcription, Genetic / radiation effects*
IF 4.011
引用数 1
リソース情報
一般微生物 JCM1333 JCM1970 JCM1973 JCM2522 JCM1338 JCM1335 JCM1336 JCM15137 JCM17812 JCM15589 JCM19549 JCM18063 JCM1318 JCM9489 JCM1305 JCM11950