RRC ID 60649
Author Gan P, Tsushima A, Hiroyama R, Narusaka M, Takano Y, Narusaka Y, Kawaradani M, Damm U, Shirasu K.
Title Colletotrichum shisoi sp. nov., an anthracnose pathogen of Perilla frutescens in Japan: molecular phylogenetic, morphological and genomic evidence.
Journal Sci Rep
Abstract Species of the fungal genus Colletotrichum are among the most devastating pathogens of agricultural crops in the world. Based on DNA sequence data (ITS, GAPDH, CHS-1, ACT, TUB2) and morphology, we revealed Colletotrichum isolates infecting the oil crop Perilla frutescens, commonly known as shiso, to represent a previously unknown species of the C. destructivum species complex and described it as C. shisoi. We found that C. shisoi appears to be able to adopt a hemibiotrophic lifestyle, characterised by the formation of biotrophic hyphae followed by severe necrotic lesions on P. frutescens, but is less virulent on Arabidopsis, compared to its close relative C. higginsianum which also belongs to the C. destructivum species complex. The genome of C. shisoi was sequenced, annotated and its predicted proteome compared with four other Colletotrichum species. The predicted proteomes of C. shisoi and C. higginsianum, share many candidate effectors, which are small, secreted proteins that may contribute to infection. Interestingly, C. destructivum species complex-specific secreted proteins showed evidence of increased diversifying selection which may be related to their host specificities.
Volume 9(1)
Pages 13349
Published 2019-9-16
DOI 10.1038/s41598-019-50076-5
PII 10.1038/s41598-019-50076-5
PMID 31527702
PMC PMC6746953
MeSH Base Sequence Colletotrichum / classification Colletotrichum / genetics* Colletotrichum / isolation & purification Colletotrichum / pathogenicity Crops, Agricultural / microbiology Fungal Proteins / genetics* Genome, Fungal / genetics* Genomics Japan Multilocus Sequence Typing Perilla frutescens / microbiology* Phylogeny Plant Diseases / microbiology Proteome / genetics
IF 4.011
Times Cited 3
General Microbes JCM31818