論文 - 詳細
| RRC ID | 60651 |
|---|---|
| 著者 | Kim BJ, Kim BR, Kook YH, Kim BJ. |
| タイトル | Potential of recombinant Mycobacterium paragordonae expressing HIV-1 Gag as a prime vaccine for HIV-1 infection. |
| ジャーナル | Sci Rep |
| Abstract |
Recombinant Mycobacterium strains such as recombinant BCG (rBCG) have received considerable attention for the HIV-1 vaccine development. Recently, we described a temperature-sensitive Mycobacterium paragordonae (Mpg) strain as a novel live tuberculosis vaccine that is safer and showed an enhanced protective effect against mycobacterial infection compared to BCG. We studied the possibility of developing a vaccine against HIV-1 infection using rMpg strain expressing the p24 antigen (rMpg-p24). We observed that rMpg-p24 can induce an increased p24 expression in infected antigen presenting cells (APCs) compared to rBCG-p24. We also observed that rMpg-p24 can induce enhanced p24 specific immune responses in vaccinated mice as evidenced by increased p24-specific T lymphocyte proliferation, gamma interferon induction, antibody production and cytotoxic T lymphocyte (CTL) responses. Furthermore, an rMpg-p24 prime and plasmid DNA boost showed an increased CTL response and antibody production compared to rBCG or rMpg alone. In summary, our study indicates that a live rMpg-p24 strain induced enhanced immune responses against HIV-1 Gag in vaccinated mice. Thus, rMpg-p24 may have potential as a preventive prime vaccine in a heterologous prime-boost regimen for HIV-1 infection. |
| 巻・号 | 9(1) |
| ページ | 15515 |
| 公開日 | 2019-10-29 |
| DOI | 10.1038/s41598-019-51875-6 |
| PII | 10.1038/s41598-019-51875-6 |
| PMID | 31664100 |
| PMC | PMC6820866 |
| MeSH | AIDS Vaccines / immunology* Animals Cell Proliferation Cytokines / biosynthesis Female Gene Products, gag / immunology* HIV Antibodies / biosynthesis HIV Infections / immunology HIV Infections / prevention & control* HIV-1 / genetics HIV-1 / immunology* Interferon-gamma / biosynthesis Mice Mice, Inbred BALB C Mycobacterium / genetics* Recombination, Genetic* T-Lymphocytes, Cytotoxic / immunology Vaccines, Synthetic / immunology |
| IF | 4.011 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM18565 |