| RRC ID |
60651
|
| 著者 |
Kim BJ, Kim BR, Kook YH, Kim BJ.
|
| タイトル |
Potential of recombinant Mycobacterium paragordonae expressing HIV-1 Gag as a prime vaccine for HIV-1 infection.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Recombinant Mycobacterium strains such as recombinant BCG (rBCG) have received considerable attention for the HIV-1 vaccine development. Recently, we described a temperature-sensitive Mycobacterium paragordonae (Mpg) strain as a novel live tuberculosis vaccine that is safer and showed an enhanced protective effect against mycobacterial infection compared to BCG. We studied the possibility of developing a vaccine against HIV-1 infection using rMpg strain expressing the p24 antigen (rMpg-p24). We observed that rMpg-p24 can induce an increased p24 expression in infected antigen presenting cells (APCs) compared to rBCG-p24. We also observed that rMpg-p24 can induce enhanced p24 specific immune responses in vaccinated mice as evidenced by increased p24-specific T lymphocyte proliferation, gamma interferon induction, antibody production and cytotoxic T lymphocyte (CTL) responses. Furthermore, an rMpg-p24 prime and plasmid DNA boost showed an increased CTL response and antibody production compared to rBCG or rMpg alone. In summary, our study indicates that a live rMpg-p24 strain induced enhanced immune responses against HIV-1 Gag in vaccinated mice. Thus, rMpg-p24 may have potential as a preventive prime vaccine in a heterologous prime-boost regimen for HIV-1 infection.
|
| 巻・号 |
9(1)
|
| ページ |
15515
|
| 公開日 |
2019-10-29
|
| DOI |
10.1038/s41598-019-51875-6
|
| PII |
10.1038/s41598-019-51875-6
|
| PMID |
31664100
|
| PMC |
PMC6820866
|
| MeSH |
AIDS Vaccines / immunology*
Animals
Cell Proliferation
Cytokines / biosynthesis
Female
Gene Products, gag / immunology*
HIV Antibodies / biosynthesis
HIV Infections / immunology
HIV Infections / prevention & control*
HIV-1 / genetics
HIV-1 / immunology*
Interferon-gamma / biosynthesis
Mice
Mice, Inbred BALB C
Mycobacterium / genetics*
Recombination, Genetic*
T-Lymphocytes, Cytotoxic / immunology
Vaccines, Synthetic / immunology
|
| IF |
4.011
|
| 引用数 |
1
|
| リソース情報 |
| 一般微生物 |
JCM18565 |
