RRC ID |
60678
|
著者 |
Timmermans ML, Picott KJ, Ucciferri L, Ross AC.
|
タイトル |
Culturing marine bacteria from the genus Pseudoalteromonas on a cotton scaffold alters secondary metabolite production.
|
ジャーナル |
Microbiologyopen
|
Abstract |
The discovery of secondary metabolites from marine microorganisms is beset by numerous challenges including difficulties cultivating and subsequently eliciting expression of biosynthetic genes from marine microbes in the laboratory. In this paper, we describe a method of culturing three species from the marine bacterial genus Pseudoalteromonas using cotton scaffold supplemented liquid media. This simple cultivation method was designed to mimic the natural behavior of some members of the genus wherein they form epibiotic/symbiotic associations with higher organisms such as sponges and corals or attach to solid structures as a biofilm. Our scaffolded cultivation is highly effective at stimulating an attachment/biofilm phenotype and causes large changes to metabolite profiles for the microbes investigated. Metabolite changes include alteration to the production levels of known molecules such as violacein, thiomarinol A, and the alterochromide and prodiginine families of molecules. Finally and critically, our technique stimulates the production of unknown compounds that will serve as leads for future natural product discovery. These results suggest our cultivation approach could potentially be used as a general strategy for the activation of silent gene clusters in marine microbes to facilitate access to their full natural product biosynthetic capacity.
|
巻・号 |
8(5)
|
ページ |
e00724
|
公開日 |
2019-5-1
|
DOI |
10.1002/mbo3.724
|
PMID |
30270573
|
PMC |
PMC6528606
|
MeSH |
Aquatic Organisms / growth & development*
Aquatic Organisms / metabolism
Bacteriological Techniques / methods*
Biological Factors / metabolism*
Cotton Fiber
Culture Media / chemistry*
Pseudoalteromonas / growth & development*
Pseudoalteromonas / metabolism
Secondary Metabolism*
|
IF |
3.142
|
引用数 |
3
|
リソース情報 |
一般微生物 |
JCM11657
JCM11046
JCM1131
JCM1002 |