Reference - Detail
| RRC ID | 60704 |
|---|---|
| Author | Gao S, Ogawa M, Takami A, Takeshita K, Kato H. |
| Title | Practical and Safe Method of Long-Term Cryopreservation for Clinical Application of Human Adipose-Derived Mesenchymal Stem Cells Without a Programmable Freezer Or Serum. |
| Journal | Cryo Letters |
| Abstract |
BACKGROUND:Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising modality for cellular therapy. However, techniques of ADSC cryopreservation, which can facilitate their clinical application, haven't been established yet. OBJECTIVE:To determine optimal conditions for ADSC cryopreservation. MATERIALS AND METHODS:We used three cryoprotectants [serum containing 10% dimethyl sulfoxide; CP-1TM (5% dimethyl sulfoxide, serum-free); Stem-CellBankerTM (dimethyl sulfoxide and serum-free)], two storage temperatures (-80°C, -150°C) and two cell densities (1 × 106, 7 × 106 cells/mL). Storage was up to 18 months using cryovials. We didn't use a rate-controlled freezer or liquid nitrogen storage. RESULTS:We found that CP-1TM was a suitable cryoprotectant. Storage at -150°C and higher cell density (7×106 cells/mL) kept the best viability of ADSCs, but storage at -80°C and a lower cell density (1×106 cells/mL) is acceptable for up to 9 months. We also confirmed large quantities of ADSCs, stored with CP-1 in a cryobag, were still viable after -150°C cryopreservation for 24 months. CONCLUSION:We have developed a safe, cost-effective way to cryopreserve ADSCs that could be used in the clinical setting. |
| Volume | 41(6) |
| Pages | 337-343 |
| Published | 2020-1-1 |
| PMID | 33990810 |
| MeSH | Cell Survival Cryopreservation* Cryoprotective Agents* / pharmacology Dimethyl Sulfoxide / pharmacology Humans Mesenchymal Stem Cells* |
| IF | 0.702 |
| Resource | |
| Cord blood stem cells for research | |