論文 - 詳細
| RRC ID | 60711 |
|---|---|
| 著者 | Imura E, Shimada-Niwa Y, Nishimura T, Hückesfeld S, Schlegel P, Ohhara Y, Kondo S, Tanimoto H, Cardona A, Pankratz MJ, Niwa R. |
| タイトル | The Corazonin-PTTH Neuronal Axis Controls Systemic Body Growth by Regulating Basal Ecdysteroid Biosynthesis in Drosophila melanogaster. |
| ジャーナル | Curr Biol |
| Abstract |
Steroid hormones play key roles in development, growth, and reproduction in various animal phyla [1]. The insect steroid hormone, ecdysteroid, coordinates growth and maturation, represented by molting and metamorphosis [2]. In Drosophila melanogaster, the prothoracicotropic hormone (PTTH)-producing neurons stimulate peak levels of ecdysteroid biosynthesis for maturation [3]. Additionally, recent studies on PTTH signaling indicated that basal levels of ecdysteroid negatively affect systemic growth prior to maturation [4-8]. However, it remains unclear how PTTH signaling is regulated for basal ecdysteroid biosynthesis. Here, we report that Corazonin (Crz)-producing neurons regulate basal ecdysteroid biosynthesis by affecting PTTH neurons. Crz belongs to gonadotropin-releasing hormone (GnRH) superfamily, implying an analogous role in growth and maturation [9]. Inhibition of Crz neuronal activity increased pupal size, whereas it hardly affected pupariation timing. This phenotype resulted from enhanced growth rate and a delay in ecdysteroid elevation during the mid-third instar larval (L3) stage. Interestingly, Crz receptor (CrzR) expression in PTTH neurons was higher during the mid- than the late-L3 stage. Silencing of CrzR in PTTH neurons increased pupal size, phenocopying the inhibition of Crz neuronal activity. When Crz neurons were optogenetically activated, a strong calcium response was observed in PTTH neurons during the mid-L3, but not the late-L3, stage. Furthermore, we found that octopamine neurons contact Crz neurons in the subesophageal zone (SEZ), transmitting signals for systemic growth. Together, our results suggest that the Crz-PTTH neuronal axis modulates ecdysteroid biosynthesis in response to octopamine, uncovering a regulatory neuroendocrine system in the developmental transition from growth to maturation. |
| 巻・号 | 30(11) |
| ページ | 2156-2165.e5 |
| 公開日 | 2020-6-8 |
| DOI | 10.1016/j.cub.2020.03.050 |
| PII | S0960-9822(20)30420-6 |
| PMID | 32386525 |
| MeSH | Animals Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Ecdysteroids / biosynthesis* Gene Expression Regulation, Developmental Insect Hormones / metabolism* Insect Proteins / metabolism* Larva / growth & development Larva / metabolism Neuropeptides / metabolism* Pupa / growth & development Pupa / metabolism Signal Transduction* |
| IF | 9.601 |
| 引用数 | 0 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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