RRC ID 60753
著者 Krausfeldt LE, Farmer AT, Castro HF, Boyer GL, Campagna SR, Wilhelm SW.
タイトル Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources in Microcystis aeruginosa NIES-843.
ジャーナル Environ Microbiol
Abstract The over-enrichment of nitrogen (N) in the environment has contributed to severe and recurring harmful cyanobacterial blooms, especially by the non-N2 -fixing Microcystis spp. N chemical speciation influences cyanobacterial growth, persistence and the production of the hepatotoxin microcystin, but the physiological mechanisms to explain these observations remain unresolved. Stable-labelled isotopes and metabolomics were employed to address the influence of nitrate, ammonium, and urea on cellular physiology and production of microcystins in Microcystis aeruginosa NIES-843. Global metabolic changes were driven by both N speciation and diel cycling. Tracing 15 N-labelled nitrate, ammonium, and urea through the metabolome revealed N uptake, regardless of species, was linked to C assimilation. The production of amino acids, like arginine, and other N-rich compounds corresponded with greater turnover of microcystins in cells grown on urea compared to nitrate and ammonium. However, 15 N was incorporated into microcystins from all N sources. The differences in N flux were attributed to the energetic efficiency of growth on each N source. While N in general plays an important role in sustaining biomass, these data show that N-speciation induces physiological changes that culminate in differences in global metabolism, cellular microcystin quotas and congener composition.
巻・号 22(6)
ページ 2419-2431
公開日 2020-6-1
DOI 10.1111/1462-2920.15032
PMID 32338427
PMC PMC9008454
MeSH Amino Acids / metabolism Ammonium Compounds / pharmacology* Biomass Microcystins / metabolism* Microcystis / drug effects* Microcystis / growth & development Microcystis / metabolism Nitrogen / pharmacology* Urea / pharmacology*
IF 4.933
引用数 0
リソース情報
藻類 NIES-843