Reference - Detail
| RRC ID | 60753 |
|---|---|
| Author | Krausfeldt LE, Farmer AT, Castro HF, Boyer GL, Campagna SR, Wilhelm SW. |
| Title | Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources in Microcystis aeruginosa NIES-843. |
| Journal | Environ Microbiol |
| Abstract |
The over-enrichment of nitrogen (N) in the environment has contributed to severe and recurring harmful cyanobacterial blooms, especially by the non-N2 -fixing Microcystis spp. N chemical speciation influences cyanobacterial growth, persistence and the production of the hepatotoxin microcystin, but the physiological mechanisms to explain these observations remain unresolved. Stable-labelled isotopes and metabolomics were employed to address the influence of nitrate, ammonium, and urea on cellular physiology and production of microcystins in Microcystis aeruginosa NIES-843. Global metabolic changes were driven by both N speciation and diel cycling. Tracing 15 N-labelled nitrate, ammonium, and urea through the metabolome revealed N uptake, regardless of species, was linked to C assimilation. The production of amino acids, like arginine, and other N-rich compounds corresponded with greater turnover of microcystins in cells grown on urea compared to nitrate and ammonium. However, 15 N was incorporated into microcystins from all N sources. The differences in N flux were attributed to the energetic efficiency of growth on each N source. While N in general plays an important role in sustaining biomass, these data show that N-speciation induces physiological changes that culminate in differences in global metabolism, cellular microcystin quotas and congener composition. |
| Volume | 22(6) |
| Pages | 2419-2431 |
| Published | 2020-6-1 |
| DOI | 10.1111/1462-2920.15032 |
| PMID | 32338427 |
| PMC | PMC9008454 |
| MeSH | Amino Acids / metabolism Ammonium Compounds / pharmacology* Biomass Microcystins / metabolism* Microcystis / drug effects* Microcystis / growth & development Microcystis / metabolism Nitrogen / pharmacology* Urea / pharmacology* |
| IF | 4.933 |
| Times Cited | 0 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-843 |