RRC ID 60758
Author Antonaru LA, Cardona T, Larkum AWD, Nürnberg DJ.
Title Global distribution of a chlorophyll f cyanobacterial marker.
Journal ISME J
Abstract Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype's distribution.
Volume 14(9)
Pages 2275-2287
Published 2020-9-1
DOI 10.1038/s41396-020-0670-y
PII 10.1038/s41396-020-0670-y
PMID 32457503
PMC PMC7608106
MeSH Antarctic Regions Arctic Regions Chlorophyll* / analogs & derivatives Chlorophyll A Cyanobacteria* / genetics Light Photosynthesis Phylogeny
IF 9.18
Times Cited 0
Algae NIES-267 NIES-593 NIES-3974