Reference - Detail
| RRC ID | 60761 |
|---|---|
| Author | Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A. |
| Title | Two coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. |
| Journal | Cell Struct Funct |
| Abstract |
We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright green fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided more reliable nuclear labeling for monitoring cell-cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.Key words: fluorescent protein, cell cycle, time-lapse imaging, flow cytometry. |
| Volume | 48(2) |
| Pages | 135-144 |
| Published | 2023-7-29 |
| DOI | 10.1247/csf.23028 |
| PMID | 37394513 |
| PMC | PMC10958192 |
| MeSH | Cell Cycle Cell Cycle Proteins* / metabolism Cell Division Color Coloring Agents* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Microscopy, Fluorescence |
| IF | 2.429 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 28 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | AzaleaB5/pRSETb (RDB18218) h2-3/pRSETb (RDB18219) tFucci(SA)5/pCS2 (RDB18220) tFucci(CA)5/pCS2 (RDB18221) |