| RRC ID |
6078
|
| Author |
Kim YW, Kato K, Hirai T, Hiwasa-Tanase K, Ezura H.
|
| Title |
Spatial and developmental profiling of miraculin accumulation in transgenic tomato fruits expressing the miraculin gene constitutively.
|
| Journal |
J Agric Food Chem
|
| Abstract |
We previously developed a transgenic tomato that expresses the miraculin gene using a constitutive promoter. In this study, we profiled the developmental and spatial accumulation of the miraculin protein and mRNA in transgenic tomato fruits. Miraculin mRNA expression was almost constant up to orange stage, and then the expression increased at red stage. The miraculin protein accumulated gradually during fruit development and reached its highest level at the overripe stage. At the red stage of fruit, miraculin protein was accumulated at the highest level in the exocarp, and similar in other fruit tissues: mesocarp, dissepiment, upper placenta, lower placenta and jelly. Moreover, the pattern of miraculin accumulation in fruit tissues was the same regardless of genetic background and position at which the miraculin gene was inserted in the genome. We also discuss suitable tomato types expressing miraculin for their commercial use.
|
| Volume |
58(1)
|
| Pages |
282-6
|
| Published |
2010-1-13
|
| DOI |
10.1021/jf9030663
|
| PMID |
20014854
|
| MeSH |
Fruit / genetics
Fruit / growth & development
Fruit / metabolism
Gene Expression Regulation, Developmental*
Lycopersicon esculentum / genetics
Lycopersicon esculentum / growth & development
Lycopersicon esculentum / metabolism*
Plant Proteins / genetics*
Plant Proteins / metabolism*
Plants, Genetically Modified / genetics
Plants, Genetically Modified / growth & development
Plants, Genetically Modified / metabolism*
|
| IF |
4.192
|
| Times Cited |
22
|
|
WOS Category
|
AGRICULTURE, MULTIDISCIPLINARY
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
|
| Resource |
| Tomato |
TOMJPF00001
TOMJPF00002(Moneymaker) |
