論文 - 詳細
| RRC ID | 60839 |
|---|---|
| 著者 | Osaka N, Kanesaki Y, Watanabe M, Watanabe S, Chibazakura T, Takada H, Yoshikawa H, Asai K. |
| タイトル | Novel (p)ppGpp0 suppressor mutations reveal an unexpected link between methionine catabolism and GTP synthesis in Bacillus subtilis. |
| ジャーナル | Mol Microbiol |
| Abstract |
In bacteria, guanosine (penta)tetra-phosphate ([p]ppGpp) is essential for controlling intracellular metabolism that is needed to adapt to environmental changes, such as amino acid starvation. The (p)ppGpp0 strain of Bacillus subtilis, which lacks (p)ppGpp synthetase, is unable to form colonies on minimal medium. Here, we found suppressor mutations in the (p)ppGpp0 strain, in the purine nucleotide biosynthesis genes, prs, purF and rpoB/C, which encode RNA polymerase core enzymes. In comparing our work with prior studies of ppGpp0 suppressors, we discovered that methionine addition masks the suppression on minimal medium, especially of rpoB/C mutations. Furthermore, methionine addition increases intracellular GTP in rpoB suppressor and this effect is decreased by inhibiting GTP biosynthesis, indicating that methionine addition activated GTP biosynthesis and inhibited growth under amino acid starvation conditions in (p)ppGpp0 backgrounds. Furthermore, we propose that the increase in intracellular GTP levels induced by methionine is due to methionine derivatives that increase the activity of the de novo GTP biosynthesis enzyme, GuaB. Our study sheds light on the potential relationship between GTP homeostasis and methionine metabolism, which may be the key to adapting to environmental changes. |
| 巻・号 | 113(6) |
| ページ | 1155-1169 |
| 公開日 | 2020-6-1 |
| DOI | 10.1111/mmi.14484 |
| PMID | 32052499 |
| MeSH | Adenosine Triphosphate / metabolism Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism DNA-Directed RNA Polymerases / genetics Gene Expression Regulation, Bacterial / genetics Guanosine Pentaphosphate / metabolism* Guanosine Triphosphate / biosynthesis* Ligases / genetics Ligases / metabolism* Methionine / metabolism* Suppression, Genetic / genetics Transcription, Genetic / genetics |
| IF | 3.649 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS861 MBS862 MBS863 MBS864 MBS865 MBS866 MBS867 MBS868 MBS869 MBS870 MBS871 MBS872 MBS873 MBS874 MBS875 MBS876 MBS877 MBS878 MBS879 MBS880 MBS881 MBS882 MBS883 MBS884 MBS885 MBS886 MBS887 MBS888 MBS889 |