論文 - 詳細
| RRC ID | 60840 |
|---|---|
| 著者 | Ohtsuka Y, Inagaki H. |
| タイトル | In silico identification and functional validation of linear cationic α-helical antimicrobial peptides in the ascidian Ciona intestinalis. |
| ジャーナル | Sci Rep |
| Abstract |
We developed a computing method to identify linear cationic α-helical antimicrobial peptides (LCAMPs) in the genome of Ciona intestinalis based on its structural and physicochemical features. Using this method, 22 candidates of Ciona LCAMPs, including well-known antimicrobial peptides, were identified from 21,975 non-redundant amino acid sequences in Ciona genome database, Ghost database. We also experimentally confirmed the antimicrobial activities of five LCAMP candidates, and three of them were found to be active in the presence of 500 mM NaCl, nearly equivalent to the salt concentration of seawater. Membrane topology prediction suggested that salt resistance of Ciona LCAMPs might be influenced by hydrophobic interactions between the peptide and membrane. Further, we applied our method to Xenopus tropicalis genome and found 11 LCAMP candidates. Thus, our method may serve as an effective and powerful tool for searching LCAMPs that are difficult to find using conventional homology-based methods. |
| 巻・号 | 10(1) |
| ページ | 12619 |
| 公開日 | 2020-7-28 |
| DOI | 10.1038/s41598-020-69485-y |
| PII | 10.1038/s41598-020-69485-y |
| PMID | 32724213 |
| PMC | PMC7387483 |
| MeSH | Amino Acid Sequence Animals Anti-Infective Agents / pharmacology Antimicrobial Cationic Peptides / chemistry* Antimicrobial Cationic Peptides / genetics Antimicrobial Cationic Peptides / metabolism Bacteria / drug effects Binding Sites Ciona intestinalis / genetics Ciona intestinalis / metabolism* Computer Simulation* Databases, Genetic Gene Expression Regulation / drug effects Genome Hemocytes / drug effects Hemocytes / metabolism Lipopolysaccharides / pharmacology Microbial Sensitivity Tests NF-kappa B / metabolism Protein Conformation, alpha-Helical RNA, Messenger / genetics RNA, Messenger / metabolism Reproducibility of Results |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C.int |