論文 - 詳細
| RRC ID | 60863 |
|---|---|
| 著者 | Ma J, Jia J, Jiang X, Xu M, Guo J, Tang T, Xu X, Wu Z, Hu B, Yao K, Li L, Tang H. |
| タイトル | gp96 Is Critical for both Human Herpesvirus 6A (HHV-6A) and HHV-6B Infections. |
| ジャーナル | J Virol |
| Abstract |
Human herpesviruses 6A and 6B (HHV-6A and HHV-6B, respectively) are two virus species in the betaherpesvirus subfamily that exhibit T cell tropism. CD46 and CD134 are the cellular receptors for HHV-6A and HHV-6B, respectively. Interestingly, the efficiency of HHV-6A/6B entry is different among different types of target cells despite similar receptor expression levels on these cells. Here, we found that the cellular factor gp96 (also known as glucose-regulated protein 94 [GRP94]) is expressed on the cell surface and interacts with viral glycoprotein Q1 (gQ1) during virus entry. gp96 cell surface expression levels are associated with the efficiency of HHV-6A and HHV-6B entry into target cells. Both loss-of-function and gain-of-function experiments indicated that gp96 plays an important role in HHV-6 infection. Our findings provide new insight into the HHV-6 entry process and might suggest novel therapeutic targets for HHV-6 infection.IMPORTANCE Although new clinical importance has been revealed for human herpesviruses 6A (HHV-6A) and 6B, much is still unknown about the life cycles of these viruses in target cells. We identified a novel cellular factor, gp96, that is critical for both HHV-6A and -6B entry into host cells. As gp96 can function as an adjuvant in vaccine development for both infectious agents and cancers, it can be a potential therapeutic target for infection by these two viruses. |
| 巻・号 | 94(13) |
| 公開日 | 2020-6-16 |
| DOI | 10.1128/JVI.00311-20 |
| PII | JVI.00311-20 |
| PMID | 32295911 |
| PMC | PMC7307172 |
| MeSH | Cell Line Fetal Blood / metabolism Herpesvirus 6, Human / metabolism* Herpesvirus 6, Human / pathogenicity Humans Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Primary Cell Culture Protein Binding Roseolovirus Infections / virology T-Lymphocytes / virology Viral Envelope Proteins / metabolism Virus Internalization |
| IF | 4.324 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |