論文 - 詳細
| RRC ID | 60918 |
|---|---|
| 著者 | Nitta N, Iino T, Isozaki A, Yamagishi M, Kitahama Y, Sakuma S, Suzuki Y, Tezuka H, Oikawa M, Arai F, Asai T, Deng D, Fukuzawa H, Hase M, Hasunuma T, Hayakawa T, Hiraki K, Hiramatsu K, Hoshino Y, Inaba M, Inoue Y, Ito T, Kajikawa M, Karakawa H, Kasai Y, Kato Y, Kobayashi H, Lei C, Matsusaka S, Mikami H, Nakagawa A, Numata K, Ota T, Sekiya T, Shiba K, Shirasaki Y, Suzuki N, Tanaka S, Ueno S, Watarai H, Yamano T, Yazawa M, Yonamine Y, Di Carlo D, Hosokawa Y, Uemura S, Sugimura T, Ozeki Y, Goda K. |
| タイトル | Raman image-activated cell sorting. |
| ジャーナル | Nat Commun |
| Abstract |
The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies. |
| 巻・号 | 11(1) |
| ページ | 3452 |
| 公開日 | 2020-7-10 |
| DOI | 10.1038/s41467-020-17285-3 |
| PII | 10.1038/s41467-020-17285-3 |
| PMID | 32651381 |
| PMC | PMC7351993 |
| MeSH | Animals Cell Separation / methods* Humans Spectrum Analysis, Raman / methods* |
| IF | 12.121 |
| 引用数 | 0 |
| リソース情報 | |
| 藻類 | NIES-48 NIES-2293 NIES-2463 NIES-3640 |