RRC ID 60955
著者 Yamamoto M, Suwa Y, Sugiyama K, Okashita N, Kawaguchi M, Tani N, Matsubara K, Nakamura A, Seki Y.
タイトル The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency.
ジャーナル J Cell Sci
Abstract The pluripotency-associated transcriptional network is regulated by a core circuitry of transcription factors. The PR domain-containing protein PRDM14 maintains pluripotency by activating and repressing transcription in a target gene-dependent manner. However, the mechanisms underlying dichotomic switching of PRDM14-mediated transcriptional control remain elusive. Here, we identified C-terminal binding protein 1 and 2 (CtBP1 and CtBP2; generically referred to as CtBP1/2) as components of the PRDM14-mediated repressive complex. CtBP1/2 binding to PRDM14 depends on CBFA2T2, a core component of the PRDM14 complex. The loss of Ctbp1/2 impaired the PRDM14-mediated transcriptional repression required for pluripotency maintenance and transition from primed to naïve pluripotency. Furthermore, CtBP1/2 interacted with the PRC2 complexes, and the loss of Ctbp1/2 impaired Polycomb repressive complex 2 (PRC2) and H3K27me3 enrichment at target genes after Prdm14 induction. These results provide evidence that the target gene-dependent transcriptional activity of PRDM14 is regulated by partner switching to ensure the transition from primed to naïve pluripotency.This article has an associated First Person interview with the first author of the paper.
巻・号 133(15)
公開日 2020-8-14
DOI 10.1242/jcs.240176
PII jcs.240176
PMID 32661086
MeSH Alcohol Oxidoreductases / genetics Co-Repressor Proteins DNA-Binding Proteins* / genetics DNA-Binding Proteins* / metabolism Gene Expression Regulation Humans Polycomb Repressive Complex 2* / metabolism RNA-Binding Proteins Transcription Factors
IF 4.573
リソース情報
ヒト・動物細胞 E14tg2a(AES0135)