Author Sato Y, Igarashi Y, Hakamata Y, Murakami T, Kaneko T, Takahashi M, Seo N, Kobayashi E.
Title Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
Journal Biochem. Biophys. Res. Commun.
Abstract Because serum albumin is specifically produced by mature hepatocytes, detection system of albumin producing cells could be a valuable tool to visualize liver regeneration or development. We have developed here an albumin enhancer/promoter-driven Alb-DsRed2 Tg rat that expresses DsRed2, having liver-specific reporter gene expression of red fluorescent protein. To study the transdifferentiation of bone marrow cells (BMCs) into albumin producing cells, BMCs from the Alb-DsRed2 Tg rat were injected into rats having acute liver damage caused by 2-acetylaminofluorene plus carbon tetrachloride and chronic liver damage by repeated administration of CCl(4). DsRed2-positive cells were generated in the recipient liver after BMC injection. The number of transdifferentiated DsRed2-positive cells in chronic liver injury model was increased comparing with that in acute injury model. We propose that the Alb-DsRed2 Tg rat is well suited to studying in vivo liver regeneration.
Volume 311(2)
Pages 478-81
Published 2003-11-14
PII S0006291X03020837
PMID 14592439
MeSH Animals Animals, Genetically Modified Bone Marrow Transplantation* Cell Transplantation Hepatitis, Chronic / genetics Hepatitis, Chronic / metabolism* Hepatitis, Chronic / surgery Liver / embryology* Liver / metabolism* Liver / surgery Liver Regeneration* Luminescent Proteins / genetics Luminescent Proteins / metabolism* Male Models, Animal* Rats / genetics Rats, Wistar Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Serum Albumin / genetics Serum Albumin / metabolism*
IF 2.559
Times Cited 32
Rats W-Tg(Alb-DsRed2)34Jmsk(strainID=528) W-Tg(Alb-DsRed2)42Jmsk(strainID=529)