RRC ID 61033
Author Furuyama S, Uehara Y, Zhang B, Baba Y, Abe S, Iwamoto T, Miura S, Saku K.
Title Genotypic Effect of ABCG1 gene promoter -257T>G polymorphism on coronary artery disease severity in Japanese men.
Journal J Atheroscler Thromb
Abstract AIM:ATP-binding cassette transporter G1 (ABCG1) is a cholesterol transporter that plays a role in cholesterol efflux in the presence of high-density lipoprotein (HDL). Moreover, HDL-mediated cholesterol efflux is increased in cultured ABCG1 overexpressed cells; however, the physiological roles of ABCG1 and its contribution to atherosclerosis in humans remain unclear.
METHODS:The effect of ABCG1 -257T>G mutation on transcription activity was determined by a reporter assay. One hundred nine Japanese men with coronary artery disease (CAD) were analyzed. ABCG1 -257T>G polymorphism was assessed by mutation-selective PCR methods to identify T/T, T/G, and G/G genotypes.
RESULTS:The reporter assay showed that ABCG1 transcription activity was significantly lower (p<0.01) in the G allele of -257T>G polymorphism compared with that in the T allele. Clinically, there were no significant differences in serum triglyceride and total, LDL, and HDL cholesterol levels, or other risk factors. Logistic regression analysis revealed significant additive and dominant effects on the frequency of patients with multi-vessel disease compared with single-vessel disease (T/T vs. T/G vs. G/G, odds ratio:2.1, p=0.027; T/T vs. T/G and. G/G, odds ratio:3.5, p=0.005).
CONCLUSION:This is the first report to show that a novel ABCG1 -257T>G promoter polymorphism influences CAD severity in Japanese men.
Volume 16(3)
Pages 194-200
Published 2009-6
DOI 10.5551/jat.e380
PMID 19556716
MeSH ATP Binding Cassette Transporter, Subfamily G, Member 1 ATP-Binding Cassette Transporters / genetics* Aged Coronary Artery Disease / epidemiology Coronary Artery Disease / genetics* Coronary Artery Disease / pathology Genotype Humans Japan Male Middle Aged Mutagenesis, Site-Directed Polymorphism, Single Nucleotide* Promoter Regions, Genetic / genetics* Transcription, Genetic
Human and Animal Cells 293T(RCB2202)