論文 - 詳細
| RRC ID | 61034 |
|---|---|
| 著者 | Tomita Y, Kawasaki T, Bilim V, Takeda M, Takahashi K. |
| タイトル | Tetrapeptide DEVD-aldehyde or YVAD-chloromethylketone inhibits Fas/Apo-1(CD95)-mediated apoptosis in renal-cell-cancer cells. |
| ジャーナル | Int J Cancer |
| Abstract |
The apoptotic machinery has been intensively investigated, and interleukin-1-beta-converting enzyme (ICE) and its homologs directly mediate apoptosis by means of their unique protease activity. Fas/Apo1 (CD95), a member of the TNF-receptor family, mediates apoptosis by binding to its ligand, which is mainly expressed on lymphocytes. Here, we investigated the expression and function of both molecules in renal-cell cancer (RCC). The expression of Fas was examined in 6 RCC cell lines by immunoblotting and all of them expressed Fas. ICE and CPP32/YAMA were also identified among the cell lines. We earlier examined ACHN cells expressing low levels of BCL-2, as well as KRC/Y cells with high levels of BCL-2. Here, we found that the anti-Fas monoclonal antibody, CH-11, induced apoptosis in a dose-dependent fashion more remarkably in ACHN cells. Pre-incubation with the tetrapeptide YVAD-chloromethyl-ketone or DEVD-aldehyde inhibited Fas-mediated apoptosis. These findings suggest that, in RCC, apoptosis is induced by lymphocytes bearing Fas-L, and that it is achieved through the proteolytic action of CPP32/YAMA and/or ICE, or another member of the ICE/ced-3 protease family. |
| 巻・号 | 68(1) |
| ページ | 132-5 |
| 公開日 | 1996-9-27 |
| DOI | 10.1002/(SICI)1097-0215(19960927)68:1<132::AID-IJC23>3.0.CO;2-C |
| PII | 10.1002/(SICI)1097-0215(19960927)68:1<132::AID-IJC23>3.0.CO;2-C |
| PMID | 8895553 |
| MeSH | Amino Acid Chloromethyl Ketones / pharmacology* Antibodies, Monoclonal / pharmacology Apoptosis / drug effects* Carcinoma, Renal Cell / pathology* Caspase 3 Caspases* Cysteine Endopeptidases Cysteine Proteinase Inhibitors / pharmacology Humans Kidney Neoplasms / pathology* Oligopeptides / pharmacology* Serpins / pharmacology Tumor Cells, Cultured Viral Proteins* fas Receptor / immunology fas Receptor / physiology* |
| IF | 5.145 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB0806) HL60(RCB0041) |