RRC ID 61107
著者 Sallam A, Mira A, Ashour A, Shimizu K.
タイトル Acetylcholine esterase inhibitors and melanin synthesis inhibitors from Salvia officinalis.
ジャーナル Phytomedicine
Abstract BACKGROUND:Salvia officinalis is a traditionally used herb with a wide range of medicinal applications. Many phytoconstituents have been isolated from S. officinalis, mainly phenolic diterpenes, which possess many biological activities.
PURPOSE:This study aimed to evaluate the ability of the phenolic diterpenes of S. officinalis to inhibit acetylcholine esterase (AChE) as well as their ability to inhibit melanin biosynthesis in B16 melanoma cells.
METHODS:The phenolic diterpenes isolated from the aerial parts of S. officinalis were tested for their effect on melanin biosynthesis in B16 melanoma cell lines. They were also tested for their ability to inhibit AChE using Ellman's method. Moreover, a molecular docking experiment was used to investigate the binding affinity of the isolated phenolic diterpenes to the amino acid residues at the active sites of AChE.
RESULTS:Seven phenolic diterpenes-sageone, 12-methylcarnosol, carnosol, 7b-methoxyrosmanol, 7a-methoxyrosmanol, isorosmanol and epirosmanol-were isolated from the methanolic extract of the aerial parts of S. officinalis. Isorosmanol showed a melanin-inhibiting activity as potent as that of arbutin. Compounds 7a-methoxyrosmanol and isorosmanol inhibited AChE activity by 50% and 65%, respectively, at a concentration of 500 µM.
CONCLUSIONS:The results suggest that isorosmanol is a promising natural compound for further studies on development of new medications which might be useful in ageing disorders such as the declining of cognitive functions and hyperpigmentation.
巻・号 23(10)
ページ 1005-11
公開日 2016-9-15
DOI 10.1016/j.phymed.2016.06.014
PII S0944-7113(16)30089-7
PMID 27444345
MeSH Acetylcholine / metabolism* Antineoplastic Agents / pharmacology Cell Line, Tumor / drug effects* Cholinesterase Inhibitors / pharmacology* Diterpenes / pharmacology* Egypt Humans Melanins / biosynthesis* Melanoma, Experimental / drug therapy* Phenols / pharmacology* Plant Extracts / pharmacology Salvia officinalis / chemistry
IF 4.268
リソース情報
ヒト・動物細胞 B16 melanoma 4A5(RCB0557)