RRC ID 61164
Author Itaya M, Sakaya N, Matsunaga S, Fujita K, Kaneko S.
Title Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium.
Journal Biosci Biotechnol Biochem
Abstract pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient. Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.
Volume 70(3)
Pages 740-2
Published 2006-3-1
DOI 10.1271/bbb.70.740
PII JST.JSTAGE/bbb/70.740
PMID 16556997
MeSH Bacillus subtilis / drug effects Bacillus subtilis / genetics* Conjugation, Genetic / drug effects* Conjugation, Genetic / physiology* Culture Media / pharmacology* Kinetics Plasmids / drug effects Plasmids / genetics*
IF 1.297
Resource
Prokaryotes B. subtilis MBS771 MBS772 MBS773