Reference - Detail
| RRC ID | 61167 |
|---|---|
| Author | Itaya M. |
| Title | Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives. |
| Volume | 76(1) |
| Pages | 180-2 |
| Published | 2012-1-1 |
| DOI | 10.1271/bbb.110528 |
| PII | JST.JSTAGE/bbb/110528 |
| PMID | 22232257 |
| MeSH | Bacillus subtilis / enzymology* Bacillus subtilis / genetics* Binding Sites Endonucleases / metabolism* Genetic Loci / genetics* Genome, Bacterial / genetics* Mutagenesis, Insertional* |
| IF | 1.516 |
| Resource | |
| Prokaryotes B. subtilis | MBS774 MBS775 MBS776 MBS777 MBS778 MBS779 MBS780 MBS781 MBS782 MBS783 |