RRC ID 61184
著者 Chirila TV, Suzuki S, Papolla C.
タイトル A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking.
ジャーナル Biotechnol Appl Biochem
Abstract Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are cross-linked. The subject of this study was the self-cross-linking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported. The hydrogels were prepared either by physical cross-linking, by cross-linking with a natural compound (genipin), or by enzymatic cross-linking. The products were comparatively characterized in regard to their synthesis and background chemical aspects, physical and optical properties, mechanical properties, secondary structure, swelling/deswelling behavior, enzymatic degradation, and compatibility as substrates for cell adhesion and proliferation. The study confirmed the advantages of the HRP-induced cross-linking, which included considerably shorter gelation times, enhanced elasticity of the resulting hydrogels, and improved cytocompatibility. Discrepancies between certain results of this investigation and those reported previously were discussed in detail.
巻・号 64(6)
ページ 771-781
公開日 2017-11-1
DOI 10.1002/bab.1552
PMID 28220960
MeSH Animals Bombyx Cell Line Cell Proliferation Cell Survival Cross-Linking Reagents / chemistry Cross-Linking Reagents / metabolism* Fibroins / biosynthesis* Fibroins / chemistry Horseradish Peroxidase / metabolism* Humans Hydrogels / chemistry Hydrogels / metabolism* Hydrogen Peroxide / chemistry Hydrogen Peroxide / metabolism Molecular Structure
IF 1.638
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)