論文 - 詳細
| RRC ID | 61184 |
|---|---|
| 著者 | Chirila TV, Suzuki S, Papolla C. |
| タイトル | A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking. |
| ジャーナル | Biotechnol Appl Biochem |
| Abstract |
Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are cross-linked. The subject of this study was the self-cross-linking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported. The hydrogels were prepared either by physical cross-linking, by cross-linking with a natural compound (genipin), or by enzymatic cross-linking. The products were comparatively characterized in regard to their synthesis and background chemical aspects, physical and optical properties, mechanical properties, secondary structure, swelling/deswelling behavior, enzymatic degradation, and compatibility as substrates for cell adhesion and proliferation. The study confirmed the advantages of the HRP-induced cross-linking, which included considerably shorter gelation times, enhanced elasticity of the resulting hydrogels, and improved cytocompatibility. Discrepancies between certain results of this investigation and those reported previously were discussed in detail. |
| 巻・号 | 64(6) |
| ページ | 771-781 |
| 公開日 | 2017-11-1 |
| DOI | 10.1002/bab.1552 |
| PMID | 28220960 |
| MeSH | Animals Bombyx Cell Line Cell Proliferation Cell Survival Cross-Linking Reagents / chemistry Cross-Linking Reagents / metabolism* Fibroins / biosynthesis* Fibroins / chemistry Horseradish Peroxidase / metabolism* Humans Hydrogels / chemistry Hydrogels / metabolism* Hydrogen Peroxide / chemistry Hydrogen Peroxide / metabolism Molecular Structure |
| IF | 1.638 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |