論文 - 詳細
| RRC ID | 61188 |
|---|---|
| 著者 | Kume A, Ito K, Ueda Y, Hasegawa M, Urabe M, Mano H, Ozawa K. |
| タイトル | A G-CSF receptor-gyrase B fusion gene: A new type of molecular switch for expansion of genetically modified hematopoietic cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
We have developed a novel system for expansion of transduced hematopoietic cells. This system involves "selective amplifier genes" encoding fusion proteins between the granulocyte colony-stimulating factor receptor (Gcr) and the estrogen receptor (Er). The GcrEr chimeric gene conferred estrogen-dependent growth ability on murine hematopoietic cells. Here, we constructed a modified "selective amplifier gene" to circumvent possible concerns with the Er/estrogen switching system. The bacterial gyrase B (Gyr) gene was fused to the Gcr gene, and the GcrGyr fusion construct was introduced into interleukin-3 (IL-3)-dependent Ba/F3 cells. The dimeric antibiotic coumermycin induced IL-3-independent growth in Ba/F3 cells expressing GcrGyr. This stimulatory effect was antagonized by an excess amount of novobiocin, a monomeric form of coumermycin. These results suggest the feasibility of using Gyr as a molecular switch to regulate a growth signal in hematopoietic cells. |
| 巻・号 | 260(1) |
| ページ | 9-12 |
| 公開日 | 1999-6-24 |
| DOI | 10.1006/bbrc.1999.0859 |
| PII | S0006-291X(99)90859-8 |
| PMID | 10381335 |
| MeSH | Aminocoumarins Animals Anti-Bacterial Agents / pharmacology Artificial Gene Fusion Cell Count Cell Culture Techniques Cell Line Chimera Coumarins / pharmacology DNA Gyrase DNA Topoisomerases, Type II / genetics* Gene Expression Regulation* Hematopoietic Stem Cells / metabolism* Mice Novobiocin / pharmacology Receptors, Granulocyte Colony-Stimulating Factor / genetics* Time Factors Transfection |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) 10T1/2(RCB0247) 32D(RCB1145) |