RRC ID 61189
著者 Omokoko MD, Pambudi S, Phanthanawiboon S, Masrinoul P, Setthapramote C, Sasaki T, Kuhara M, Ramasoota P, Yamashita A, Hirai I, Ikuta K, Kurosu T.
タイトル A highly conserved region between amino acids 221 and 266 of dengue virus non-structural protein 1 is a major epitope region in infected patients.
ジャーナル Am J Trop Med Hyg
Abstract The immune response to dengue virus (DENV) infection generates high levels of antibodies (Abs) against the DENV non-structural protein 1 (NS1), particularly in cases of secondary infection. Therefore, anti-NS1 Abs may play a role in severe dengue infections, possibly by interacting (directly or indirectly) with host factors or regulating virus production. If it does play a role, NS1 may contain epitopes that mimic those epitopes of host molecules. Previous attempts to map immunogenic regions within DENV-NS1 were undertaken using mouse monoclonal Abs (MAbs). The aim of this study was to characterize the epitope regions of nine anti-NS1 human monoclonal Abs (HuMAbs) derived from six patients secondarily infected with DENV-2. These anti-NS1 HuMAbs were cross-reactive with DENV-1, -2, and -3 but not DENV-4. All HuMAbs bound a common epitope region located between amino acids 221 and 266 of NS1. This study is the first report to map a DENV-NS1 epitope region using anti-DENV MAbs derived from patients.
巻・号 91(1)
ページ 146-55
公開日 2014-7-1
DOI 10.4269/ajtmh.13-0624
PII ajtmh.13-0624
PMID 24778195
PMC PMC4080554
MeSH Amino Acid Sequence Animals Antibodies, Monoclonal / biosynthesis Antigens, Viral / chemistry Antigens, Viral / immunology* Conserved Sequence Dengue / immunology* Dengue / virology Dengue Virus / classification Dengue Virus / genetics Dengue Virus / immunology* Epitopes / chemistry Epitopes / immunology* Gene Expression Host-Pathogen Interactions Humans Mice Molecular Sequence Data Peptide Mapping Recombinant Proteins / genetics Recombinant Proteins / immunology Serotyping Viral Nonstructural Proteins / genetics Viral Nonstructural Proteins / immunology*
IF 2.126
リソース情報
ヒト・動物細胞 SP2/0-Ag14(RCB0209)