論文 - 詳細
| RRC ID | 61189 |
|---|---|
| 著者 | Omokoko MD, Pambudi S, Phanthanawiboon S, Masrinoul P, Setthapramote C, Sasaki T, Kuhara M, Ramasoota P, Yamashita A, Hirai I, Ikuta K, Kurosu T. |
| タイトル | A highly conserved region between amino acids 221 and 266 of dengue virus non-structural protein 1 is a major epitope region in infected patients. |
| ジャーナル | Am J Trop Med Hyg |
| Abstract |
The immune response to dengue virus (DENV) infection generates high levels of antibodies (Abs) against the DENV non-structural protein 1 (NS1), particularly in cases of secondary infection. Therefore, anti-NS1 Abs may play a role in severe dengue infections, possibly by interacting (directly or indirectly) with host factors or regulating virus production. If it does play a role, NS1 may contain epitopes that mimic those epitopes of host molecules. Previous attempts to map immunogenic regions within DENV-NS1 were undertaken using mouse monoclonal Abs (MAbs). The aim of this study was to characterize the epitope regions of nine anti-NS1 human monoclonal Abs (HuMAbs) derived from six patients secondarily infected with DENV-2. These anti-NS1 HuMAbs were cross-reactive with DENV-1, -2, and -3 but not DENV-4. All HuMAbs bound a common epitope region located between amino acids 221 and 266 of NS1. This study is the first report to map a DENV-NS1 epitope region using anti-DENV MAbs derived from patients. |
| 巻・号 | 91(1) |
| ページ | 146-55 |
| 公開日 | 2014-7-1 |
| DOI | 10.4269/ajtmh.13-0624 |
| PII | ajtmh.13-0624 |
| PMID | 24778195 |
| PMC | PMC4080554 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal / biosynthesis Antigens, Viral / chemistry Antigens, Viral / immunology* Conserved Sequence Dengue / immunology* Dengue / virology Dengue Virus / classification Dengue Virus / genetics Dengue Virus / immunology* Epitopes / chemistry Epitopes / immunology* Gene Expression Host-Pathogen Interactions Humans Mice Molecular Sequence Data Peptide Mapping Recombinant Proteins / genetics Recombinant Proteins / immunology Serotyping Viral Nonstructural Proteins / genetics Viral Nonstructural Proteins / immunology* |
| IF | 2.126 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SP2/0-Ag14(RCB0209) |