Reference - Detail
| RRC ID | 611 |
|---|---|
| Author | Tambasco-Studart M, Titiz O, Raschle T, Forster G, Amrhein N, Fitzpatrick TB. |
| Title | Vitamin B6 biosynthesis in higher plants. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Vitamin B6 is an essential metabolite in all organisms. It can act as a coenzyme for numerous metabolic enzymes and has recently been shown to be a potent antioxidant. Plants and microorganisms have a de novo biosynthetic pathway for vitamin B6, but animals must obtain it from dietary sources. In Escherichia coli, it is known that the vitamin is derived from deoxyxylulose 5-phosphate (an intermediate in the nonmevalonate pathway of isoprenoid biosynthesis) and 4-phosphohydroxy-l-threonine. It has been assumed that vitamin B6 is synthesized in the same way in plants, but this hypothesis has never been experimentally proven. Here, we show that, in plants, synthesis of the vitamin takes an entirely different route, which does not involve deoxyxylulose 5-phosphate but instead utilizes intermediates from the pentose phosphate pathway, i.e., ribose 5-phosphate or ribulose 5-phosphate, and from glycolysis, i.e., dihydroxyacetone phosphate or glyceraldehyde 3-phosphate. The revelation is based on the recent discovery that, in bacteria and fungi, a novel pathway is in place that involves two genes (PDX1 and PDX2), neither of which is homologous to any of those involved in the previously doctrined E. coli pathway. We demonstrate that Arabidopsis thaliana has two functional homologs of PDX1 and a single homolog of PDX2. Furthermore, and contrary to what was inferred previously, we show that the pathway appears to be cytosolic and is not localized to the plastid. Last, we report that the single PDX2 homolog is essential for plant viability. |
| Volume | 102(38) |
| Pages | 13687-92 |
| Published | 2005-9-20 |
| DOI | 10.1073/pnas.0506228102 |
| PII | 0506228102 |
| PMID | 16157873 |
| PMC | PMC1224648 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carbon-Nitrogen Lyases Cytoplasm / enzymology* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Fungal Proteins / genetics Fungal Proteins / metabolism Fungi / enzymology Fungi / genetics Glycolysis / physiology Molecular Sequence Data Nitrogenous Group Transferases / genetics Nitrogenous Group Transferases / metabolism* Pentose Phosphate Pathway / physiology Pentosephosphates / genetics Pentosephosphates / metabolism Vitamin B 6 / biosynthesis* Vitamin B 6 / genetics |
| IF | 9.412 |
| Times Cited | 128 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pst00965 |