Reference - Detail
| RRC ID | 61205 |
|---|---|
| Author | Osakabe K, Wada N, Miyaji T, Murakami E, Marui K, Ueta R, Hashimoto R, Abe-Hara C, Kong B, Yano K, Osakabe Y. |
| Title | Genome editing in plants using CRISPR type I-D nuclease. |
| Journal | Commun Biol |
| Abstract |
Genome editing in plants has advanced greatly by applying the clustered regularly interspaced short palindromic repeats (CRISPRs)-Cas system, especially CRISPR-Cas9. However, CRISPR type I-the most abundant CRISPR system in bacteria-has not been exploited for plant genome modification. In type I CRISPR-Cas systems, e.g., type I-E, Cas3 nucleases degrade the target DNA in mammals. Here, we present a type I-D (TiD) CRISPR-Cas genome editing system in plants. TiD lacks the Cas3 nuclease domain; instead, Cas10d is the functional nuclease in vivo. TiD was active in targeted mutagenesis of tomato genomic DNA. The mutations generated by TiD differed from those of CRISPR/Cas9; both bi-directional long-range deletions and short indels mutations were detected in tomato cells. Furthermore, TiD can be used to efficiently generate bi-allelic mutant plants in the first generation. These findings indicate that TiD is a unique CRISPR system that can be used for genome engineering in plants. |
| Volume | 3(1) |
| Pages | 648 |
| Published | 2020-11-6 |
| DOI | 10.1038/s42003-020-01366-6 |
| PII | 10.1038/s42003-020-01366-6 |
| PMID | 33159140 |
| PMC | PMC7648086 |
| MeSH | Clustered Regularly Interspaced Short Palindromic Repeats Deoxyribonucleases / genetics* Gene Editing* Genetic Engineering* Genome, Plant* Solanum lycopersicum / genetics* |
| IF | 4.165 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 15 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Tomato | TOMJPF00004 (Ailsa Craig) |
| Human and Animal Cells | 293T(RCB2202) |