| RRC ID |
61205
|
| Author |
Osakabe K, Wada N, Miyaji T, Murakami E, Marui K, Ueta R, Hashimoto R, Abe-Hara C, Kong B, Yano K, Osakabe Y.
|
| Title |
Genome editing in plants using CRISPR type I-D nuclease.
|
| Journal |
Commun Biol
|
| Abstract |
Genome editing in plants has advanced greatly by applying the clustered regularly interspaced short palindromic repeats (CRISPRs)-Cas system, especially CRISPR-Cas9. However, CRISPR type I-the most abundant CRISPR system in bacteria-has not been exploited for plant genome modification. In type I CRISPR-Cas systems, e.g., type I-E, Cas3 nucleases degrade the target DNA in mammals. Here, we present a type I-D (TiD) CRISPR-Cas genome editing system in plants. TiD lacks the Cas3 nuclease domain; instead, Cas10d is the functional nuclease in vivo. TiD was active in targeted mutagenesis of tomato genomic DNA. The mutations generated by TiD differed from those of CRISPR/Cas9; both bi-directional long-range deletions and short indels mutations were detected in tomato cells. Furthermore, TiD can be used to efficiently generate bi-allelic mutant plants in the first generation. These findings indicate that TiD is a unique CRISPR system that can be used for genome engineering in plants.
|
| Volume |
3(1)
|
| Pages |
648
|
| Published |
2020-11-6
|
| DOI |
10.1038/s42003-020-01366-6
|
| PII |
10.1038/s42003-020-01366-6
|
| PMID |
33159140
|
| PMC |
PMC7648086
|
| MeSH |
Clustered Regularly Interspaced Short Palindromic Repeats
Deoxyribonucleases / genetics*
Gene Editing*
Genetic Engineering*
Genome, Plant*
Solanum lycopersicum / genetics*
|
| IF |
4.165
|
| Resource |
| Tomato |
TOMJPF00004 (Ailsa Craig) |
| Human and Animal Cells |
293T(RCB2202) |
