論文 - 詳細
RRC ID | 61209 |
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著者 | Sato N, Wang S, Li L, Okabe K, Hashimoto M, Yaginuma H, Mikoshiba K, Uchiyama Y, Uetsuki T, Yoshikawa K, Milligan CE, Oppenheim RW. |
タイトル | A novel strategy for introducing exogenous bcl-2 into neuronal cells: the Cre/loxP system-mediated activation of bcl-2 for preventing programmed cell death using recombinant adenoviruses. |
ジャーナル | Mol Cell Neurosci |
Abstract |
We have established a novel strategy for introducing exogenous Bcl-2 into neuronal cells that is mediated by Cre/loxP recombination using recombinant adenoviral vectors. An on/off-switching cassette for Bcl-2 (CALNLbcl-2) was designed to express Bcl-2 by recombinase Cre-mediated excisional deletion of a spacer DNA flanked by a pair of loxP sites. Exogenous Bcl-2 was clearly induced in PC12 cell lines carrying CALNLbcl-2 after infection with recombinant adenovirus producing recombinase Cre (AxCANCre). Dual infection with both AxCANCre and a recombinant adenovirus bearing CALNLbcl-2 showed efficient delivery of exogenous Bcl-2 into a hybrid motoneuronal cell line and primary chicken spinal motoneurons. The delivery of foreign Bcl-2 promoted survival of motoneurons in medium either containing or lacking trophic support. Thus, this strategy for delivery of exogenous Bcl-2 will be useful for studying neuronal death as well as for introducing foreign genes into postmitotic neurons under the control of recombinase Cre. |
巻・号 | 12(1-2) |
ページ | 65-78 |
公開日 | 1998-9-1 |
DOI | 10.1006/mcne.1998.0703 |
PII | S1044-7431(98)90703-5 |
PMID | 9770341 |
MeSH | Adenoviridae* Adenoviridae Infections / genetics Animals Apoptosis / physiology Chick Embryo DNA Replication Gene Expression Regulation, Viral Genes, Viral / physiology Humans Integrases / metabolism* Kidney / cytology Neurons / cytology* Neurons / enzymology Neurons / virology PC12 Cells Proto-Oncogene Proteins c-bcl-2 / genetics* Rats Recombinant Fusion Proteins / physiology Transduction, Genetic* Viral Proteins* |
IF | 3.182 |
リソース情報 | |
ヒト・動物細胞 | PC-12(RCB0009) |