論文 - 詳細
| RRC ID | 61219 |
|---|---|
| 著者 | Kobayashi K, Matsuura E, Liu Q, Furukawa J, Kaihara K, Inagaki J, Atsumi T, Sakairi N, Yasuda T, Voelker DR, Koike T. |
| タイトル | A specific ligand for beta(2)-glycoprotein I mediates autoantibody-dependent uptake of oxidized low density lipoprotein by macrophages. |
| ジャーナル | J Lipid Res |
| Abstract |
beta(2)-Glycoprotein I (beta(2)-GPI) is a major antigen for antiphospholipid antibodies (Abs) present in patients with the antiphospholipid syndrome (APS). We previously reported that beta(2)-GPI specifically binds to oxidized low density lipoprotein (oxLDL), but not to native low density lipoprotein (LDL). In the present study, a ligand specific for beta(2)-GPI, oxLig-1, was purified from the extracted lipids of oxLDL. The structure of oxLig-1 was shown to be identical to that of synthesized 7-ketocholesteryl-9-carboxynonanoate by mass spectroscopy and nuclear magnetic resonance analyses. Both purified and synthesized oxLig-1 were recognized by beta(2)-GPI and subsequently by anti-beta(2)-GPI auto-Abs, either in enzyme-linked immunosorbent assay (ELISA) or in ligand blot analysis. Binding of liposomes containing oxLig-1 (oxLig-1-liposomes) to mouse macrophages, J774A.1 cells, was relatively low, as compared with that of phosphatidylserine (PS)-liposomes. In contrast, binding of oxLig-1-liposomes was enhanced more than 10-fold in the presence of both beta(2)-GPI and an anti-beta(2)-GPI auto-Ab (WB-CAL-1), derived from (NZW x BXSB) F1 mouse, an animal APS model. Anti-beta(2)-GPI auto-Abs derived from APS patients with episodes of arterial thrombosis were detected in ELISA, using a solid phase oxLig-1 complexed with beta(2)-GPI. We suggest that autoimmune atherogenesis linked to beta(2)-GPI interaction with oxLDL and Abs may be present in APS. |
| 巻・号 | 42(5) |
| ページ | 697-709 |
| 公開日 | 2001-5-1 |
| PII | S0022-2275(20)31631-X |
| PMID | 11352976 |
| MeSH | Animals Antiphospholipid Syndrome / complications* Antiphospholipid Syndrome / physiopathology Arteriosclerosis / etiology Arteriosclerosis / immunology Autoantibodies / immunology Autoantibodies / metabolism* Cell Line Endocytosis / physiology Enzyme-Linked Immunosorbent Assay Glycoproteins / immunology Glycoproteins / metabolism* Humans Ligands Lipoproteins, LDL / chemistry Lipoproteins, LDL / immunology Lipoproteins, LDL / metabolism* Liposomes / chemistry Liposomes / metabolism Macrophages / metabolism* Magnetic Resonance Spectroscopy Methylation Mice Molecular Structure Phospholipids / metabolism Thrombosis / etiology Thrombosis / immunology beta 2-Glycoprotein I |
| IF | 4.483 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | J774.1(RCB0434) |