RRC ID 61233
著者 Okuyama T, Ishihara S, Sato H, Rumi MA, Kawashima K, Miyaoka Y, Suetsugu H, Kazumori H, Cava CF, Kadowaki Y, Fukuda R, Kinoshita Y.
タイトル Activation of prostaglandin E2-receptor EP2 and EP4 pathways induces growth inhibition in human gastric carcinoma cell lines.
ジャーナル J Lab Clin Med
Abstract The effect of prostaglandin E2 (PGE2) on the proliferation of gastric cancer cells is still unclear. PGE2 receptors are divided into four subtypes - EP1, EP2, EP3, and EP4 - which are coupled to three different intracellular signal-transduction systems. Stimulation of EP2 and EP4 is linked with cyclic adenosine 3', 5'-monophosphate (cAMP)-dependent protein kinase A (PKA). In some human gastric cancer cells, PGE2 has been suggested to have an antiproliferative effect by way of increased cAMP production. Expression of EP2 and EP4 in human gastric carcinoma cells, however, has not been examined. We examined the expression of EP2 and EP4 and the antiproliferative effects of specific EP2 and EP4 agonists on four different human gastric cancer cell lines. Our data clarified that all the cell lines investigated in this study expressed EP2 and EP4 and that the specific agonists of these receptors induced growth inhibition with an accompanying increase in cAMP production. In summary, gastric cancer cells have EP2 and EP4 receptors, and their selective activation is linked with the decreased cell proliferation.
巻・号 140(2)
ページ 92-102
公開日 2002-8-1
DOI 10.1067/mlc.2002.125784
PII S0022214302000239
PMID 12228765
MeSH Cell Division / drug effects Cell Division / physiology Cyclic AMP / metabolism Cyclooxygenase Inhibitors / pharmacology Dinoprostone / pharmacology Gene Expression Regulation, Neoplastic / drug effects Humans Indomethacin / pharmacology RNA, Messenger / analysis Receptors, Prostaglandin E / agonists Receptors, Prostaglandin E / genetics* Receptors, Prostaglandin E / metabolism* Receptors, Prostaglandin E, EP1 Subtype Receptors, Prostaglandin E, EP2 Subtype Receptors, Prostaglandin E, EP3 Subtype Receptors, Prostaglandin E, EP4 Subtype Stomach Neoplasms* Tumor Cells, Cultured / cytology Tumor Cells, Cultured / metabolism
リソース情報
ヒト・動物細胞 MKN7(RCB0999) MKN45(RCB1001)