RRC ID 61240
Author Lin YY, Chen CY, Chuang TY, Lin Y, Liu HY, Mersmann HJ, Wu SC, Ding ST.
Title Adiponectin receptor 1 regulates bone formation and osteoblast differentiation by GSK-3β/β-catenin signaling in mice.
Journal Bone
Abstract Adiponectin and its receptors are expressed in bone marrow-derived osteoblasts. Previous studies in vivo and in vitro have produced controversial results. The purpose of this study was to use porcine adiponectin receptor 1 transgenic mice (pAdipoR1) as a model to evaluate the role of AdipoR1 on bone physiology at different ages. pAdipoR1 transgenic mice had higher bone mineral density than wild-type mice in both genders at 56 weeks of age. The bone volume and trabecular number, measured by micro-computed tomography (μCT) was significantly greater in transgenic than in wild-type female mice at both 8 and 56 weeks of age. ELISA analysis revealed that both serum osteocalcin and osteoprotegerin (OPG) were significantly increased in 8-week old pAdipoR1 transgenic mice of both genders. Furthermore, serum OPG was elevated at 32 and 56 weeks of age in female and male pAdipoR1 transgenic mice. Serum TRAP5b concentration was reduced in 8 and 56 weeks old male pAdipoR1 mice compared with wild-type male mice. Knock-down of AdipoR1 significantly decreased gene expression of osteocalcin, OPG, alkaline phosphatase and msh homeobox 2 and the mineralization in MC3T3-E1 cells and mesenchymal stem cells. In addition, pathscan analysis and real-time PCR analysis suggest AdipoR1 regulates osteoblast differentiation through GSK-3 β and β-Catenin signaling. Consequently, the lack of AdipoR1 impaired osteoblast differentiation and bone formation. We conclude that AdipoR1 is a critical factor for the osteoblast differentiation and bone homeostasis.
Volume 64
Pages 147-54
Published 2014-7-1
DOI 10.1016/j.bone.2014.03.051
PII S8756-3282(14)00125-2
PMID 24713193
MeSH 3T3 Cells Animals Base Sequence Bone Development / physiology* Cell Differentiation / physiology* DNA Primers Enzyme-Linked Immunosorbent Assay Female Glycogen Synthase Kinase 3 / metabolism* Glycogen Synthase Kinase 3 beta Mice Mice, Transgenic Osteoblasts / cytology* Osteoblasts / enzymology Osteoblasts / metabolism Real-Time Polymerase Chain Reaction Receptors, Adiponectin / physiology* Signal Transduction X-Ray Microtomography beta Catenin / metabolism*
IF 4.147
Human and Animal Cells MC3T3-E1(RCB1126)