RRC ID 61248
Author Mikami Y, Tsuda H, Akiyama Y, Honda M, Shimizu N, Suzuki N, Komiyama K.
Title Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner.
Journal J Bone Miner Metab
Abstract Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various cells. Poly(P) concentrations of 1 and 0.5 mg/mL yielded high levels of mineralization in ROS17/2.8 osteoblast cells. Similarly, Poly(P) induced mineralization in cell types expressing alkaline phosphatase (ALP), namely, ATDC5 and MC3T3-E1, but not in CHO, C3H10T1/2, C2C12, and 3T3-L1 cells. Furthermore, forced expression of ALP caused Poly(P)-induced mineralization in CHO cells. These results suggest that ALP determines Poly(P)-induced mineralization in a cell-type independent manner.
Volume 34(6)
Pages 627-637
Published 2016-11-1
DOI 10.1007/s00774-015-0719-6
PII 10.1007/s00774-015-0719-6
PMID 26475372
MeSH 3T3-L1 Cells Alkaline Phosphatase / biosynthesis* Alkaline Phosphatase / genetics Animals CHO Cells Calcification, Physiologic / drug effects* Calcification, Physiologic / genetics Cricetinae Cricetulus Gene Expression Regulation, Enzymologic / drug effects* Gene Expression Regulation, Enzymologic / physiology Mice Organ Specificity / drug effects Organ Specificity / physiology Osteoblasts / cytology Osteoblasts / enzymology* Polyphosphates / pharmacology* Rats
IF 2.297
Human and Animal Cells MC3T3-E1(RCB1126)