RRC ID 61310
Author Kim J, Han C, Jo W, Kang S, Cho S, Jeong D, Gho YS, Park J.
Title Cell-Engineered Nanovesicle as a Surrogate Inducer of Contact-Dependent Stimuli.
Journal Adv Healthc Mater
Abstract Heterotypic interactions between cells are crucial in various biological phenomena. Particularly, stimuli that regulate embryonic stem cell (ESC) fate are often provided from neighboring cells. However, except for feeder cultures, no practical methods are identified that can provide ESCs with contact-dependent cell stimuli. To induce contact-dependent cell stimuli in the absence of living cells, a novel method that utilizes cell-engineered nanovesicles (CNVs) that are made by extruding living cells through microporous membranes is described. Protein compositions of CNVs are similar to their originating cells, as well as freely diffusible and precisely scalable. Treatment of CNVs produced from three different stromal cells successfully induces the same effect as feeder cultures. The results suggest that the effects of CNVs are mainly mediated by membrane-associated components. The use of CNVs might constitute a novel and efficient tool for ESC research.
Volume 6(17)
Published 2017-9-1
DOI 10.1002/adhm.201700381
PMID 28643483
MeSH Animals Cell Communication* Cell Differentiation Cell Engineering / methods* Cell Line Cell Self Renewal Embryonic Stem Cells Hematopoiesis Mice, Inbred C57BL Nanoparticles / chemistry* Neurons / cytology
IF 6.27
Resource
Human and Animal Cells MC3T3-E1(RCB1126)